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Team:Newcastle/Agar plates
From 2010.igem.org
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==Protocol== | ==Protocol== | ||
| - | * Dissolve the materials in 800 ml of distilled water. ( Note : The agar will not dissolve in | + | * Dissolve the materials in 800 ml of distilled water. ( Note : The agar will not dissolve in distilled water. ) |
| - | * After most of the materials are dissolved, top up the | + | * After most of the materials are dissolved, top up the final volume to 1 liter with distilled water. |
* Autoclave the mixture. | * Autoclave the mixture. | ||
* After autoclaving, allow the mixture to cool down before adding the appropriate antibiotic. | * After autoclaving, allow the mixture to cool down before adding the appropriate antibiotic. | ||
Latest revision as of 14:14, 26 October 2010
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LB agar plates
Materials required
The materials mentioned below is used to make up 1 liter of the standard LB agar plates:
- 10 g of tryptoptan
- 5 g of yeast
- 5 g of NaCl
- 15 g of agar
The materials mentioned is used to make up 1 liter of LB starch agar plates:
- 10 g of tryptoptan
- 5 g of yeast
- 5 g of NaCl
- 15 g of agar
- 10 g of starch
Protocol
- Dissolve the materials in 800 ml of distilled water. ( Note : The agar will not dissolve in distilled water. )
- After most of the materials are dissolved, top up the final volume to 1 liter with distilled water.
- Autoclave the mixture.
- After autoclaving, allow the mixture to cool down before adding the appropriate antibiotic.
- Pour appropriately 25 ml of the mixture into petri dishes.
Note: Aseptic technique must be employed after the autoclave part.
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