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Team:MIT mammalian Bone
From 2010.igem.org
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| + | <b> Background </b> | ||
| + | |||
| + | The goal of this portion of the project was to synthetically differentiate stem cells into bone; this would act as the 'output' for our final system. Since stem cells can by tricky to genetically engineer, we chose to build our circuit in human endothelial kidney (HEK) cells. In the final system, HEK cells will secrete a diffusible, osteogenic morphogen to differentiate co-cultured stem cells. We used Bone Morphogenetic Protein 2 (BMP2) as a | ||
<a href="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg" target="_blank"> <img width=600px src="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg"> </a> | <a href="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg" target="_blank"> <img width=600px src="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg"> </a> | ||
| + | <b> References </b> | ||
| + | |||
| + | 1. Hogan, B. L. (1996) Harvey Lect. 92, 83-98 | ||
| + | 2. Katagiri, T., Yamaguchi, A., Komaki, M., Ab, E., Takahashi, N., Ikeda, T., Rosen, V., Wozney, J. M., Fujisawa-Sehara, A., Suda, T. (1994) J. Cell Biol. 127, 1755-1766 | ||
| + | 3. Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, Wang EA, Tanaka H, Omura S, Suda T (1990) . Biochem Biophy Res Commun 172:295–299 | ||
| + | 4. Yamaguchi A, Katagiri T, Ikeda T, Wozney JM, Rosen V, Wang EA, Kahn AJ, Suda T, Yoshiki S (1991) . J Cell Biol 113:681– | ||
| + | |||
</td></table> | </td></table> | ||
Revision as of 12:49, 26 October 2010
iGEM 2010
Bone Formation |
Background
The goal of this portion of the project was to synthetically differentiate stem cells into bone; this would act as the 'output' for our final system. Since stem cells can by tricky to genetically engineer, we chose to build our circuit in human endothelial kidney (HEK) cells. In the final system, HEK cells will secrete a diffusible, osteogenic morphogen to differentiate co-cultured stem cells. We used Bone Morphogenetic Protein 2 (BMP2) as a
References
1. Hogan, B. L. (1996) Harvey Lect. 92, 83-98
2. Katagiri, T., Yamaguchi, A., Komaki, M., Ab, E., Takahashi, N., Ikeda, T., Rosen, V., Wozney, J. M., Fujisawa-Sehara, A., Suda, T. (1994) J. Cell Biol. 127, 1755-1766
3. Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, Wang EA, Tanaka H, Omura S, Suda T (1990) . Biochem Biophy Res Commun 172:295–299
4. Yamaguchi A, Katagiri T, Ikeda T, Wozney JM, Rosen V, Wang EA, Kahn AJ, Suda T, Yoshiki S (1991) . J Cell Biol 113:681–
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