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Team:Alberta/Notebook/TransformingCells
From 2010.igem.org
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This method was adapted from a method used by Justin Fedor. | This method was adapted from a method used by Justin Fedor. | ||
| - | Preparation of transformation buffer - July 6, 2010 | + | *Preparation of transformation buffer - July 6, 2010 |
| - | Preparation of cells - July 10, 2010 | + | *Preparation of cells - July 10, 2010 |
| - | Preparing competent cells - July 11, 2010 | + | *Preparing competent cells - July 11, 2010 |
| - | Lyophilization - July 13, 2010 | + | *Lyophilization - July 13, 2010 |
| - | Transformation of FD cells - July 14, 2010 | + | *Transformation of FD cells - July 14, 2010 |
| - | Preparation of cells 2 - July 30, 2010 | + | *Preparation of cells 2 - July 30, 2010 |
| - | Lyophilization - August 2, 2010 | + | *Lyophilization - August 2, 2010 |
| - | Transformation - August 5, 2010 | + | *Transformation - August 5, 2010 |
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{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Revision as of 04:54, 26 October 2010
Transforming Cells
Transformation typically requires expensive equipment and storing competent cells is difficult for many high schools who do not have -80C freezers. We tried a few methods of transforming cells and making them competent to attempt to solve this problem in our kit.
TSS Transformation Protocol
The TSS transformation was tested. The procedure is listed here.
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution
http://www.pnas.org/content/86/7/2172.abstract
- Acquistion of reagents- June 3, 2010
- Preparation of buffer - June 7, 2010
- Cell preparation - June 7, 2010
- Competency - June 10, 2010
Rubidium chloride competent cells and lyophilization
This method was adapted from a method used by Justin Fedor.
- Preparation of transformation buffer - July 6, 2010
- Preparation of cells - July 10, 2010
- Preparing competent cells - July 11, 2010
- Lyophilization - July 13, 2010
- Transformation of FD cells - July 14, 2010
- Preparation of cells 2 - July 30, 2010
- Lyophilization - August 2, 2010
- Transformation - August 5, 2010
