Team:SDU-Denmark/project-t

From 2010.igem.org

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Note that although the bacteria will be stationary in our system, since they are glued to the inner surface of the flowchannel, our construct in reality confers phototactic ability to E. coli.
Note that although the bacteria will be stationary in our system, since they are glued to the inner surface of the flowchannel, our construct in reality confers phototactic ability to E. coli.
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[[image:Phototaxis_mechanism.png‎ | 650px]]
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[[image:Phototaxis_mechanism.png‎ | 650px | thumb | A. The SRII rhodopsin has not yet been activated. Note that CheA is active by default, continuosly autophosphorylating itself, and cycling back to it's unphosphorylated state by transfering the phosphoryl group to CheY. High levels of CheY-P will induce tumbling motion in the flagella. Note also that CheZ continuously dephosphorylates CheY.<br>
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B. SRII is now hit with a photon, causing conformational change of the entire complex, and shutting of CheA. The Inactivation of CheA halts production of CheY-P, and CheZ rapidly dephosphorylates the remaining CheY-P, resulting in a reduced frequency of tumbling.]]
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''A. The SRII rhodopsin has not yet been activated. Note that CheA is active by default, continuosly autophosphorylating itself, and cycling back to it's unphosphorylated state by transfering the phosphoryl group to CheY. High levels of CheY-P will induce tumbling motion in the flagella. Note also that CheZ continuously dephosphorylates CheY.''<br>
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''B. SRII is now hit with a photon, causing conformational change of the entire complex, and shutting of CheA. The Inactivation of CheA halts production of CheY-P, and CheZ rapidly dephosphorylates the remaining CheY-P, resulting in a reduced frequency of tumbling.''<br><br>
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====BioBrick design====
====BioBrick design====

Revision as of 10:18, 26 October 2010