Team:Alberta/Kit

From 2010.igem.org

(Difference between revisions)
(→GENOMIKON)
(→GENOMIKON)
Line 29: Line 29:
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is:
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is:
*Fast: Parts can be added to a growing construct in 7 minutes.
*Fast: Parts can be added to a growing construct in 7 minutes.
-
*Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''.
+
*Efficient: It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''.
-
*Equipment-It does not require centrifugation or any other expensive equipment inherently.
+
*Equipment: It does not require centrifugation or any other expensive equipment inherently.
</p>
</p>
'''The Kit'''<p>
'''The Kit'''<p>
The other limitations were solved by using innovative protocols and products:
The other limitations were solved by using innovative protocols and products:
-
*Equipment-Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
+
*Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
-
*Easily Shipped-We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
+
*Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
-
*Inexpensive-Based on our [[Team:Alberta/Distribution analysis|distribution analysis]] the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.</p>
+
*Inexpensive: Based on our [[Team:Alberta/Distribution analysis|distribution analysis]] the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.</p>
<p>
<p>
'''[picture of kit buddi]'''
'''[picture of kit buddi]'''

Revision as of 03:10, 26 October 2010

TEAM ALBERTA

GENOMIKON

The Idea

GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs.

To work GENOMIKON must be:

  • Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
  • Fast
  • Efficicent
  • Easily shipped
  • Inexpensive

The Innovation

The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, BioBytes 2.0. The BioByte 2.0 standard solved most of the problems.

BioBytes 2.0 is:

  • Fast: Parts can be added to a growing construct in 7 minutes.
  • Efficient: It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
  • Equipment: It does not require centrifugation or any other expensive equipment inherently.

The Kit

The other limitations were solved by using innovative protocols and products:

  • Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
  • Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
  • Inexpensive: Based on our distribution analysis the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.

[picture of kit buddi]

Safety

GENOMIKON was designed with safety in mind and as such it has many safety features built in.

  • A common lab strain of E. coli DH5α is used. This strain has been used safely in labs around the world and was designed to be safe.
  • Proper safety equipment is not required but will be recommended to further decrease the risks of contamination.
  • The kit will teach safety education to the students.

Reception

When we tested our kit with high school students from the Edmonton area it had a warm reception. They had great things to say about the educational aspect and the scientfic aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on real world trials.

Retrieved from "http://2010.igem.org/Team:Alberta/Kit"