Protocol/7

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(New page: {{Team:Alberta/Head}} {{Team:Alberta/navbar|project=selected}} {{Team:Alberta/beginLeftSideBar}} {{Team:Alberta/endLeftSideBar}} {{Team:Alberta/beginRightSideBar}} {{Team:Alberta/endRig...)
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Protocol 7: Gel Extraction
Protocol 7: Gel Extraction
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What you will need:
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Reagents:
* Clean scalpel
* Clean scalpel
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* Eppendorf tube
* Eppendorf tube
* Gel extraction kit
* Gel extraction kit
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* 55oC water bath
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* 55<sup>o</sup>C water bath
* Vortex
* Vortex
* Microfuge
* Microfuge
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Procedure
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Procedure:
* Place the gel on the transilluminator. Put on face shield and gloves.
* Place the gel on the transilluminator. Put on face shield and gloves.
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* Pry out the gel ‘cube’ and place it in an eppendorf tube.
* Pry out the gel ‘cube’ and place it in an eppendorf tube.
* Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
* Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
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[[Team:Alberta/Notebook/protocols| Back]]
[[Team:Alberta/Notebook/protocols| Back]]
{{Team:Alberta/endMainContent}}
{{Team:Alberta/endMainContent}}

Revision as of 01:18, 26 October 2010

TEAM ALBERTA

Protocol 7: Gel Extraction


Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].


Back