Team:Newcastle/Meetings/26 March 2010

Formal meeting - 26th March 2010

• Room: CBCB; Start: 11 am
• Chair: Jannetta, Minutes: Rachel, Computer: Harsh
• Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
• Apologies: Phil (Holiday), Jem and Matt
• Approval of agenda and minutes - Approved

4. Research Feedback

Properties of concrete and surface tension (Zoltan and Steven)

• High compressive strength low tensile strength
• Steal added good for temp change
• CaCO₃ – similar to concrete; expand same as concrete... might crack like concrete
• Spider silk like steal- reduce possibility of cracking
• Glue- no many papers- expansion may not be a problem?
• Silk alkali resistant
• Capillary properties of concrete change over time- in project we consider small rapid
• Cement water ratio and surface area and environment
• Where to look for the cracks? Crack pattern

Microinjection (Zoltan and Steven)

• Simple issue
• Not correct term
• Swimming Bacillus
• Oxygen gradient big selling points

Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)

• Test in plasmids- Find good plasmid
• Integrate into genome 
• Recombination site... Find!!!
• amyE site amylase with insert can’t break down amylase

Nutrients (Harsh and Rachel)

• Bacteria algae growth living in concrete
• Only activate feed bacillus
• Two hybrid
• Biofilms exclude other not algae
• Antifungals, antialgaes, antibiotic

Sporulation, germination and secretion control (Alan and Da)

• SpoA – last years team sporulation control, maybe turn off completely
• Nutrient availability
• KinA
• Inducible promoter
• Glue out of bug

Quorum sensing (Younus and Phil)

• CompX and cff
• Two-component system comP

Kill switch (Richard and Jannetta)

• Why ? Don’t want?- not die after job fill new cracks
• Nuclease
• Phil masEF
• Skf SbB
• If linear DNA works

Glue, CaCo3 and spider silk (Zoltan and Jannetta)

• Bacteria that produce CaCo3 from urea
• How secrete spider silk long complex proteins – how can we make it happen in bacillus
• Secretion systems

Other points

• Linear DNA – Richard

Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-

  |5. Action points

|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-

   |1. Individual iGEM applications

|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-

        |2. Visas/Passport update

|- |Harsh- May June apply |- |Richard visa waiver |-

        |3. Wiki transfer

|-

              |1. Clarify contents of identified pages

|-

              |2. Id Ethics page 

|- |Research page |- |Safety page |- |entify other pages required |-

              |3. Ideas for layout / theme

|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-

    |4. Travel plans

|-

              |1. Flights

|- |Need to sort out who is going |-

              |2. Accomodation

|- |Need to sort out who is going |-

        |5. Funding

|- |Harsh has made draft letter |- |Main chemical company – free stuff ?

|-

        |6. Advisors/additional team members - civil engineer/graphics designer?

|- |Still looking –Neil,Jen |-

        |7. Tutorials

|- |Sequentia and Clone manager- basics |- |Modelling part two |-

        |8. What next?

|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-

Time line discussion

• Need someone else on time line.
• 16th of June Lab induction
• Deadlines soon
• Change flight deadline – not 31st

Other business

• Pdf mark up tools – PDF exchange viewer, foxit, keeps mark-up
• Making dropbox use better

Items for next agenda

• Need wiki transferred by next meeting
• Review of flow chart if done

Next meeting

• Thursday early as possible – email to organise
• Chair: Da, Minutes: Alan, Computer: Younus