Team:Alberta/Achievements

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TEAM ALBERTA

• HOME
• AT A GLANCE
• PROJECT
  • Overview

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  • BioBytes 2.0

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  • Software

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  • Modelling

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  • The Kit

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  • Kit Components
• ACHIEVEMENTS
  • Overview

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  • Medal Requirements

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  • Media
• HUMAN PRACTICES
  • Overview

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  • Distribution Analysis

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  • High School

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  • DiscoverElle Visit

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  • Safety
• PARTS
• TIMELINE & PROTOCOLS
  • Overview

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  • General Protocols

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  • Reusable Plates

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  • Base Plasmids

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  • Creating Parts

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  • Transforming Cells

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  • Anchor

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  • Beads

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  • Optimizations

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  • Software
• TEAM

Contents 1 WWW.GENOMIKON.CA 2 BRONZE MEDAL 3 SILVER MEDAL 4 Team Alberta in the Media

WWW.GENOMIKON.CA

This is the online lab manual and educational resource for the Genomikon Kit

BRONZE MEDAL

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Design and Document BioBrick Parts and DNA submission

We characterized and uploaded 31 new parts to the Registry of Parts. These were:

• 11 Plasmids
• 15 ORFs
• 4 Linkers

All submitted parts have been verified as the correct size using test digests and gel electrophoresis. Sequencing files are posted on the Parts Registry as advanced sequence analyses. Beyond our additions to the Registry of Parts we also have parts that will work with Genomikon that could not be uploaded to the registry because they are created from oligonucleotides.

SILVER MEDAL

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BioByte 2.0 Method<p> The BioByte 2.0 system was engineered to allow for the fast and efficient assembly of BioBytes into large constructs. This was shown to work in multiple on-bead assemblies which included the construction of a 12kBp long octamer and construction of multiple plasmids that were successfully transformed into E. coli. The successful construction and transformation of E. coli with plasmids created using the BioByte 2.0 method was a major accomplishment of this years project.

All of our parts have been tested in PCR's and shown to give a product of the correct size

Demonstration of New Parts<p> All of the 31 parts uploaded to the Registry of Parts are new and conform to the BioBrick standard. All of our RFP plasmids work as we designed them to and can be used to rapidly create BioByte 2.0 parts. These BioBytes can subsequently be used to assemble DNA constructs.

Characterization of New Parts<p> All of the 31 new parts that were uploaded to the Registry of Parts were tested extensively as this was required for their operation in the BioByte 2.0 system as documented. All the parts were checked using PCR and gel electrophoresis to ensure proper size. The untransformed constructs that were made, including the octamer, were shown using gel electrophoresis to be of expected length. All of the parts that worked in the transformation experiments have been shown to work in vivo. This also shows that they all work within the BioByte 2.0 assembly standard.

Listing out how this fufills the judging criteria?

(going by last year's example? [1])

Plus the special/area prizes

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The requirements to earn a Gold are:

1. Register the team, have a great summer, and have fun attending the Jamboree.
2. Successfully complete and submit a Project Summary form.
3. Create and share a Description of the team's project via the iGEM wiki (see TUDelft 2008 for a great example).
4. Present a Poster and Talk at the iGEM Jamboree (watch the Heidelberg 2008 video for a great example).
5. Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts
6. Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts.
7. Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected.
8. Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts (see [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] for an exemplar).
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     • • • • Plus:
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9. Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.
   • • AND/OR
10. Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
    • • AND/OR
11. Develop and document a new technical standard that supports the (i) design of BioBrick Parts or Devices, or (ii) construction of BioBrick Parts or Devices, or (iii) characterization of BioBrick Parts or Devices, or (iv) analysis, modeling, and simulation of BioBrick Parts or Devices, or (v) sharing BioBrick Parts or Devices, either via physical DNA or as information via the internet.
    • • AND/OR
12. Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

Team Alberta in the Media

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• Edmonton Sun - October 25, 2010
  • http://www.edmontonsun.com/news/edmonton/2010/10/24/15813611.html