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Team:UPO-Sevilla/Notebook/10 08
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<h1>October, 8th</h1> | <h1>October, 8th</h1> | ||
| - | <h2> | + | <h2>Assembly Team</h2> |
| - | <p>Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. | + | <p>Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. UPO16+3/3T5 was confirmed.</p> |
| - | <p>Minipreps of UPO4, 5 and 11/1C3 inocula | + | <p>Minipreps and digestion of UPO4, 5 and 11/1C3 inocula (2h, 37ºC). Samples were run in a 0.8% agarose gel. We had to repeat these digestions because there were little DNA quantity. Digestion again.</p> |
<p>Transformation of 13+3/3T5 ligations in DHTα.</p> | <p>Transformation of 13+3/3T5 ligations in DHTα.</p> | ||
| - | <p>Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified using GFX. Ligation of 1+2+7+3. </p> | + | <p>Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified by using GFX. Ligation of 1+2+7+3. </p> |
<p>12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX. | <p>12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX. | ||
</p> | </p> | ||
Revision as of 15:25, 27 October 2010
October, 8th
Assembly Team
Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. UPO16+3/3T5 was confirmed.
Minipreps and digestion of UPO4, 5 and 11/1C3 inocula (2h, 37ºC). Samples were run in a 0.8% agarose gel. We had to repeat these digestions because there were little DNA quantity. Digestion again.
Transformation of 13+3/3T5 ligations in DHTα.
Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified by using GFX. Ligation of 1+2+7+3.
12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX.
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