Team:Heidelberg/Notebook/Material

From 2010.igem.org

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(Primer)
(Materials)
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Please find our Primers & Oligos on an [https://2010.igem.org/Team:Heidelberg/Notebook/Material/Primer extra subpage].
Please find our Primers & Oligos on an [https://2010.igem.org/Team:Heidelberg/Notebook/Material/Primer extra subpage].
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 +
== Instruments ==
 +
 +
The following is a list of all used Instruments.
 +
{| class="wikitable sortable"  border="0" style="text-align: left"
 +
|+ align="top, left"|'''table 6''': Used Instruments.
 +
! Description || Name || Firm
 +
| Table Centrifuge || Microfuge'''®''' 18 Centrifuge || Beckman Coulter<sup>TM</sup>
 +
| Table Centrifuge  || MicrofugeR 22'''®''' Centrifuge  || Beckman Coulter<sup>TM</sup>
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| Centrifuge  ||  Allegra X-12 || Beckman Coulter<sup>TM</sup>
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|  Shaker || VORTEXGENIER 2  || Scientific Industries, SI<sup>TM</sup>
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|  Heating plate (magnet stirrer) || MR Hei-Standard  || Heidolph
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|  Heatblock || QBD4  || Grant
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|  Heatblock (shakeing function) || Thermomixer comfort  || Eppendorf
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|- style="background-color:#9BDDFF;"
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| PCR-machine  || MyCycler<sup>TM</sup> thermo cycler  || BioRad
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|  UV-chamber ||  Transluminator || Vilber Lourmat
 +
| Scale (fine)  || Pioneer<sup>TM</sup> PA114C  || OHAUS
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|  Scale ||  Pioneer<sup>TM</sup> PA4101C || OHAUS
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| Fridge  || KTP 1750 Premium || Liebherr
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| Freezer  || GP 1366 Premium  || Liebherr
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| -86 Freezer  ||  -86°C Ultralow Freezer || NUAIRE<sup>TM</sup>
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|  Hood || Tischabzug  || Wesemann'''®''' Laboreinrichtung
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| Draw-off pump  || Vacuhand control  || Vacubrand
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| Incubator  || HT Multitron Version 2  || INFORS
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| Incubator (cell culture)  || HERAcell 150  || Thermo electron coorperation
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| Hood (cell culture)  || HERAsafe  || Thermo electron coorperation
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| Plate reader  || Infinite M200  || TECAN
 +
| FACS  || Cytomics FC 500 MPL  || Beckman Coulter<sup>TM</sup>
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| Microscope  || CKX41  || Olympus
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| Computer  ||  || Sun Microsystems
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| UV/Visivle Spectrometer  || Ultrospec 3300 pro  || Amersham Biosciences
 +
| Photometer  || NanoDrop'''®''' ND-1000 Spectrophotometer  || peQLab Biotechnologie GmbH
 +
| Photometer  || NanoVue<sup>TM</sup>  || General Electric
 +
| Ice Machine  || MF22  || SCOTSMAN'''®'''
 +
| Gel electrophoresis chamber  || Mupid'''®'''-One  || Advance
 +
| pipetting robot  || QIAcube<sup>TM</sup>  || QIAGEN
 +
| Wide field microscope  || Nikon Eclipse 90i  || Nikon
 +
| PCR machine  || MyCycler thermal cycler  || BIO-RAD
 +
| Real-time PCR machine  || StepOnePlus<sup>TM</sup> Real-time PCR System  || Applied Biosystems<sup>TM</sup>
 +
|- style="background-color:#9BDDFF;"
 +
|}
{{:Team:Heidelberg/Pagemiddle}}
{{:Team:Heidelberg/Pagemiddle}}

Revision as of 21:31, 25 October 2010

Materials

Kits

table 1: Used kits.
KitsSupplierCatalog Number
HiSpeed® Plasmid Maxi Kit (25)QIAGEN12663
CompactPrep Plasmid Maxi Kit (25)QIAGEN12763
RNeasy® Mini Kit (25)QIAGEN74106
QIAquick® Miniprep Kit (250)QIAGEN27106
QIAquick® Gel Extraction Kit (250)QIAGEN28706
QIAquick® Nucleotid Removal Kit (250)QIAGEN28306
QIAquick® PCR Purification Kit (250)QIAGEN28106
GeneMorph® II EZC Clone Domain Mutagenesis KitStratagene200552-5

Marker

table 2: Used DNA ladders for gel electrophoresis.
NameSupplierRange
GeneRuler™ High Range DNA LadderFermentas10,171-48,502 bp
GeneRuler™ 1 kb Plus DNA LadderFermentas75-20,000 bp

Enzymes

table 3: Used NEB restriction enzymes.
NameNumber/IDNEB buffer1NEB buffer2NEB buffer3NEB buffer4BSA
AflII2750%100%25%100%x
AgeI1100%50%10%75%
ApaI-25%50%0%100%x
AseInot75%100%not
AvrII-100%100%50%100%
BamHI275%100%100%100%x
BamHI-75%100%100%100%x
BclI-50%100%100%75%
BglI-50%75%100%50%
BglII13 10% 75% 100% 10%
BsrGI- 25% 100% 10% 100%x
DpnI 6 100% 100% 75% 100%
DpnI - 100% 100% 75% 100%
EcoRI - 100% 100% 100% 100%
EcoRV - 50% 75% 100% 50% x
FseI - 100% 75% 0% 100%
HindIII- 10 50% 100% 10% 50%
HindIII-50% 100% 10% 50%
KpnI 11 100% 75% 0% 50% x
MfeI-HF™- 75% 50% 10% 100%
NarI 26 100% 75% 75% 100%
NcoI 12 100% 100% 100% 100%
NdeI - 75% 100% 75% 100%
NheI - 100% 100% 10% 100% x
NotI 14 0% 50% 100% 25%
NsiI - 10% 75% 100% 25%
PciI - 50% 75% 100% 50% x
PstI 15 75% 75% 100% 50%
SacI 16 100% 50% 10% 100%
ScaI 18 notnot100%not
SfcI 19 75% 50% 10% 100% x
SfcI-75% 50% 10% 100% x
SpeI - 75% 100% 25% 100% x
SphI - 100% 100% 50% 100%
SspI - 50% 100% 50% 50%
XbaI 23 0% 100% 75% 100% x
XbaI 56 0% 100% 75% 100% x
XhoI24 75% 100% 100% 100% x
XmaI25 25% 50% 0% 100% x

NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X

Bacteria

table 4: Used E. coli strains.
NamePurposeReference
DH5αAmplification of PlasmidsInvitrogen
SCS110Amplification of unmethylated PlasmidsStratagene
Top10Amplification of PlasmidsInvitrogen

Media and Antibiotics

  • LB (10 g tryptone, 5 g yeast extract, 10 g sodium chloride, ad 1 L ddH2O)
table 5: Used antibiotics.
AntibioticFinal Concentration Solvent
Ampicillin100 µg/mlddH2O
Chloramphenicol25 µg/ml100% Ethanol
Kanamycin50 µg/mlddH2O

Primers & Oligos

Please find our Primers & Oligos on an extra subpage.

Instruments

The following is a list of all used Instruments.

table 6: Used Instruments.
Description Name Firm Table Centrifuge Microfuge® 18 Centrifuge Beckman CoulterTM Table Centrifuge MicrofugeR 22® Centrifuge Beckman CoulterTM Centrifuge Allegra X-12 Beckman CoulterTM Shaker VORTEXGENIER 2 Scientific Industries, SITM Heating plate (magnet stirrer) MR Hei-Standard Heidolph Heatblock QBD4 Grant Heatblock (shakeing function) Thermomixer comfort Eppendorf
PCR-machine MyCyclerTM thermo cycler BioRad UV-chamber Transluminator Vilber Lourmat Scale (fine) PioneerTM PA114C OHAUS Scale PioneerTM PA4101C OHAUS Fridge KTP 1750 Premium Liebherr Freezer GP 1366 Premium Liebherr -86 Freezer -86°C Ultralow Freezer NUAIRETM Hood Tischabzug Wesemann® Laboreinrichtung Draw-off pump Vacuhand control Vacubrand Incubator HT Multitron Version 2 INFORS Incubator (cell culture) HERAcell 150 Thermo electron coorperation Hood (cell culture) HERAsafe Thermo electron coorperation Plate reader Infinite M200 TECAN FACS Cytomics FC 500 MPL Beckman CoulterTM Microscope CKX41 Olympus Computer Sun Microsystems UV/Visivle Spectrometer Ultrospec 3300 pro Amersham Biosciences Photometer NanoDrop® ND-1000 Spectrophotometer peQLab Biotechnologie GmbH Photometer NanoVueTM General Electric Ice Machine MF22 SCOTSMAN® Gel electrophoresis chamber Mupid®-One Advance pipetting robot QIAcubeTM QIAGEN Wide field microscope Nikon Eclipse 90i Nikon PCR machine MyCycler thermal cycler BIO-RAD Real-time PCR machine StepOnePlusTM Real-time PCR System Applied BiosystemsTM

Contents