Team:Newcastle/Meetings/1 July 2010

Formal Meeting - 1st July 2010

• Agenda

Roll call

Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.

Action points from last week

• Wiki changes have been made.
• Letter to Andrew Young is not finished - someone needs to be in charge of this.
• Civil engineer funding is not done yet.
• Flights: plan is to get there on Thursday about 4-5 p.m.

Research feedback - end of crack signalling, quorum sensing

• Oxygen limitation triggers subtilin production, ResD and E.
• Need subtilin immunity brick too spaIFEG.
• Subtilin receptor was done in 2008 by Newcastle spaRK.
• Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
• We need to get the strain with these genes. ATCC6633.

Lab feedback

• Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
• Urea media, grown overnight, If the urea is used, there will be a colour change.

• Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.

• Phil and Younus, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.

Logo and T-shirts

• Finalised.
• University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
• CBCB logo needs to be added. Ask Jeff or Joanna.

Visas

• Younus still hasn't filled in the visa-waiver form.

Funding

• Andrew Young letter needs to be finished and checked tomorrow.

Timeline

• Could be made more detailed now.
• Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
• Spider silk next? It's on our logo so we should do it.

Action points

• Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
• All: Andrew Young letter needs to be finished and checked tomorrow.

Next meeting

• Next Wednesday, 3 p.m.
• Chair: Harsh, Minutes: Alan, Computer: Phil