Team:SDU-Denmark/project-i

The Idea

Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [http://microfluids.engin.brown.edu/Breuer_Papers/Journals/Small2008_Bacterial_Pump.pdf] we have decided to attempt construction of a similar system. Our approach centers on E. coli that will be modified to hyper-express flagella, in an attempt to increase force generation potential. These bacteria will be attached to a surface, and their flagella should therefor generate flow in the surrounding liquid.

Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.

Both sub-project will contribute new parts to the registry.