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Team:Calgary/24 October 2010
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Final touches being added to the wiki over the next few days. Team pages and new header put into place. | Final touches being added to the wiki over the next few days. Team pages and new header put into place. | ||
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| + | <u>Emily</u> | ||
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| + | Today I trabsformed and plated our remianing plasmid switches (getting our final parts and constructs into the Regitsry accepted psB1C3 vector). I also prepared competant cells containing the I0500-I13504 plasmid in the psB1C3 vector. These cells were then used to transform malESSDel and malE31SSDel expression constructs from the Betton labs so that we can see if malE31SSDel and malESSDel work the way that we expect them to. I also transformed the Lethbridge team's construct into these cells in order to assay cytoplasmic protein msfolding. I also ran gels of my PCRs from yesterday in order to confirm that my construction of I0500-B0034 with the part that we got from Lethbridge was successful as well as to confirm that we were able to get ibpAB into the psBA1C3 vector. | ||
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Revision as of 23:18, 26 October 2010
Sunday October 24, 2010
Patrick
Final touches being added to the wiki over the next few days. Team pages and new header put into place.
Emily
Today I trabsformed and plated our remianing plasmid switches (getting our final parts and constructs into the Regitsry accepted psB1C3 vector). I also prepared competant cells containing the I0500-I13504 plasmid in the psB1C3 vector. These cells were then used to transform malESSDel and malE31SSDel expression constructs from the Betton labs so that we can see if malE31SSDel and malESSDel work the way that we expect them to. I also transformed the Lethbridge team's construct into these cells in order to assay cytoplasmic protein msfolding. I also ran gels of my PCRs from yesterday in order to confirm that my construction of I0500-B0034 with the part that we got from Lethbridge was successful as well as to confirm that we were able to get ibpAB into the psBA1C3 vector.
