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Team:Newcastle/5 July 2010
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==Protocol== | ==Protocol== | ||
| - | * Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3. | + | * Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3 to prepare stocks. |
==Inference== | ==Inference== | ||
*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction. | *Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 15:05, 25 October 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (rfp).
Materials
- Miniprep of pMutin4.
- Miniprep of pSB1AT3.
Protocol
- Set up a overnight culture of colonies transformed with pMutin4 and pSB1AT3 to prepare stocks.
Inference
- Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
   
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