Team:Sheffield/Notebook

1. Self healing pipes - a method for pipes to 'recover' from damage using bacterial biofilms.

2. Water desalination - how we can use specialised bacteria to lower the salt content of sea water to make it drinkable.

3. Lowering nitrate levels - using bacteria to lower nitrate levels in rivers, helping to promote healthy living conditions for fish

4. Carbon, nitrogen and phosphorus ratios - alteration of these using bacteria to inhibit growth of pathogenic bacteria

5. "Bactoshave!" - the bacterial shaving system... (patent pending)

Wednesday 23rd June - A small meeting between Thomas Leach, Narmada Herath and Steven Garrett comparing 2006, 2007, 2008 and 2009 IGEM projects from other universities. Particular interest was taken in microbial fuel cells, as these may be adapted for desalination. We're hoping to look further into exploiting this to desalinate samples of water possibly in cartridge form

The projects using search and destroy bacteria and self healing pipes were also considered in some detail. These will be discussed further during the next meeting.

The Wikis we noted were: 2009: - Lethbridge, City College San Francisco and Missouri (bacterial fuel cells) - Columbia (sea water salt detection) - Wisconsin (increased resistance to salt) - Brussels (E.coli glue) - Newcastle (ion sequestering)

2008: - Heidelberg (targetting biofilms in pipes) - Brown (electrical reporting system) - Lethbridge (Search and destroy harmful hydrocarbons) - Calgary (pathogen killing machine) - Illinois (cholera detection) - Newcastle (B. subtillus as a biosensor) - Edinburgh (producing starch from cellulose and biomass)

2007: - Columbia (biosensor) - Glasgow (fuel cell) - MIT (clearing mercury contamination) - Turkey (pH dependent metal ion transporter) - South Utah (cyanide biosensor) - Brown (Lead contamination)

2006: - Edinburgh (sensing arsenic in drinking water)

Monday 28th June - 10 week IGEM placements officially begin. Our next meeting is planned aiming to narrow down the project to something realistic and attainable during the 10 weeks.

The session was attended by all the undergrads and Andy. A twitter account was set up - already Steve is addicted. Catherine received lots of our quizzing emails. We emailed Xia Huang, regarding the paper Cao, X., et al. "A New Method for Water Desalination Using Microbial Desalination Cells." Environmental science & technology 43.18 (2009):7148-7152. We emailed Environmentalbiotech.com to find out more information about their grease eradication system and also Sheffield Forgemasters about their waste desposal methods.

A few ideas about order and layout of the wiki were talked about during the meeting, with some designs being drawn up for a logo. We've decided to go with 'Drip & Drop'... or 'Drop & Drip' the water droplets as team mascots (still a BIG ongoing debate between a few members).

For desalination we decided an in-situ method would be most realistic. We would probably require well characterised halo-tolerant bacteria that can form a biofilm on an electrode. The system in theory would have a middle chamber where salt water is placed. Two flanking chambers would contain opposite electrodes sepatated from the middle chamber by selectively permeable membranes to either anions or cations.

A broad area we looked at on this day was the destruction/removal of pollutants/pathogens from water supply. Our potential targets included iron, lead, nitrates, pharmaceuticals, fats, oils, grease, sewage, various pathogens e.g. cholera and toxins.

Final note - team lunch of pizza at Rise @ the Hallamshire is definitely something worth considering again.

Tuesday 29th June - Today we got a guided tour of our labs for the very first time courtesy of Quaiser Sheikh. Exciting stuff!

We looked at the proteins TonB and ExbB for potential use in our desalination cell. The fuel cell desalination idea was adapted to just a desalination cell consisting of 3 chambers without electrodes, with chambers following the same order as before, but this time with a chloride sequestering bacteria in once chamber and a sodium sequestering bacteria in the other. This still needs some research to work out how realistic this idea is. We liked Newcastle's project from 2009 and considered how Bac-Man might be able to aid our project this year in this area.

Removing nitrate in sewage treatment plants may also hold potential. We would be looking at ways to lower the amount of nitrate released by the plants using novel bacteria with a consortium of enzymes to 'mop up' the excess. The process could also be energy productive, opposed to energy consuming (where aeration is concerned).

Finally we're looking into completing the work done by the Sheffield 2008 team. This was a project aiming to detect the presence of cholera, however due to complications the team could not complete their project.

Lunch today - sandwiches in the sun in Weston Park. Tom, Narmada and Caroline revealed their vegetarianism to the rest of the group *gasp*.

Wednesday 30th June - 09:30 start to try tie up loose ends on initial ideas before the 14:00 meeting with team advisors.

Okay, so the 09:30 start went slightly less to plan as we'd hoped, ranging from a few members arriving bright and breezy to those not so bright and breezy ones (Matt "12pm" ford). That aside the morning consisted of drawing up ideas for parts needed for desalination (e.g. ion channels or the 'bacman system' created by Newcastle University). Further work on Sheffield iGEM 2008 was made. We're now in the hope of modifying the system with an easier reporter (e.g. those used by Cambridge 2009's E.chromi). Thi could potentially give rise to a universal pathogen detector, with various colour outputs depending on the pathogen present in E.coli. There is potential to engineer this system in B.subtillus as a spin-off project as a proof of principle to produce a dry, portable biosensor for pathogens.

Following lunch at Interval, we met up with advisors fearing a grilling. Overall however, our hard work appears to have paid off, with all ideas appearing realistic within the 10 weeks (except using fuel cells for desalination, which Greg is having a further look into for us). We're now going to research further the ion channels which might make desalination possible. Improving Sheffield 2008's project was very popular at the meeting - many ideas have now been born from this, so full steam ahead at looking for biobricks tomorrow.

Post-grilling we took some nice photos to go up on here and visited the molecular biology and biotechnology labs with Quaiser. We finished off the day with a round of drinks at the University Arms where we talked about the 1000000 keyrings Steve's ordering, Nii's gloves and performing our Jamboree presentation through the medium of dance.

Quotes of the day:

"Mmm yeah... but try thinking of a good idea instead Krish" - Andy

"I don't get the joke" - Tom

"I think of other stuff in the shower too, but I probably shouldn't talk about it" - Nii.

Trivia: Sheffield's team members are probably quite rare - 3 of the 4 veggies (andy too) are lefties!

Thursday 1st July - the team say "White rabbit" for good luck. The aim's to look at the catalogue of parts, decide what we need and a create suitable logo. We should check out getting Visas for the Jamboree too. Potential for some Wiki work today too - it's looking a bit scruffy at the moment.

notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.