Team:Brown/Protocols
From 2010.igem.org
(Difference between revisions)
Line 7: | Line 7: | ||
*[[:Team:Brown/Protocols/PCR|PCR]] | *[[:Team:Brown/Protocols/PCR|PCR]] | ||
PCR Cleanup/Purification | PCR Cleanup/Purification | ||
- | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification (page 19)]] | + | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] |
Gel Extraction | Gel Extraction | ||
- | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction (page 25)]] | + | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] |
Ligations | Ligations | ||
*[[:Team:Brown/Protocols/Ligation|Ligation]] | *[[:Team:Brown/Protocols/Ligation|Ligation]] | ||
Line 19: | Line 19: | ||
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] | *[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] | ||
Cell competence protocols | Cell competence protocols | ||
- | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]] | + | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]] |
- | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] | + | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]] |
Minipreps | Minipreps | ||
- | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook (pages 22-23)]] | + | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]] |
Revision as of 08:37, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps