Team:Brown/Protocols

From 2010.igem.org

(Difference between revisions)
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*[[:Team:Brown/Protocols/PCR|PCR]]
*[[:Team:Brown/Protocols/PCR|PCR]]
PCR Cleanup/Purification
PCR Cleanup/Purification
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*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification (page 19)]]
+
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]]
Gel Extraction
Gel Extraction
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*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction (page 25)]]
+
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]]
Ligations
Ligations
*[[:Team:Brown/Protocols/Ligation|Ligation]]
*[[:Team:Brown/Protocols/Ligation|Ligation]]
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*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]]
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]]
Cell competence protocols
Cell competence protocols
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*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]]
+
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]]
-
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]]
+
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]]
Minipreps
Minipreps
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*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook (pages 22-23)]]
+
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]

Revision as of 08:37, 25 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

PCR

PCR Cleanup/Purification

Gel Extraction

Ligations

Restriction digests

Overnights

Transformations

Cell competence protocols

Minipreps