Team:Harvard/flavor/results
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Revision as of 04:52, 25 October 2010
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Results
YFP-tagged Miraculin and Brazzein are successfully expressed
In order to confirm that the Miraculin and Brazzein are able to be expressed in E. Coli we attached a YFP-2x tag sequence to the termini of both proteins. The proteins were placed under an IPTG-expressible promoter and used spectrophotometry to determine the level of YFP fluorescence against a baseline, untagged protein. Figure 1 shows relative-fluorescence at times post induction. In all circumstances the levels of YFP-fluorescence increased
Figure 1 click to enlarge ![]() |
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