Team:Brown/Protocols
From 2010.igem.org
(Difference between revisions)
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*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | ||
Transformations | Transformations | ||
- | *[[:Team:Brown/Protocols/Transformation|Transformation]] | + | *[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] |
Cell competence protocols | Cell competence protocols | ||
*[[Media:Protocol_Quick_CaCl2_competence.pdf]] | *[[Media:Protocol_Quick_CaCl2_competence.pdf]] |
Revision as of 04:42, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook. PCR
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols