Team:Brown/Protocols

From 2010.igem.org

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Cell competence protocols
Cell competence protocols
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[[Media|Protocol_Quick_CaCl2_competence.pdf}}
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*[[Media|Protocol_Quick_CaCl2_competence.pdf]]

Revision as of 04:32, 25 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

  • PCR

PCR

  • Ligations

Ligation

  • Restriction digests

Restriction Digest Overnights

Transformations

Cell competence protocols

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