Team:Brown/Protocols

From 2010.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
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Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
-
**PCR
+
*PCR
-
*[[:Team:Brown/Protocols/PCR|PCR]]
+
[[:Team:Brown/Protocols/PCR|PCR]]
-
**Ligations
+
*Ligations
-
*[[:Team:Brown/Protocols/Ligation|Ligation]]
+
[[:Team:Brown/Protocols/Ligation|Ligation]]
-
**Restriction digests
+
*Restriction digests
-
*[[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest]]
+
[[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest]]
-
*Overnights
+
Overnights
-
**[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
+
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
-
*Transformations
+
Transformations
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**[[:Team:Brown/Protocols/Transformation|Transformation]]
+
*[[:Team:Brown/Protocols/Transformation|Transformation]]
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*Cell competence protocols
+
Cell competence protocols
 +
[[Media|Protocol_Quick_CaCl2_competence.pdf}}

Revision as of 04:31, 25 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

  • PCR

PCR

  • Ligations

Ligation

  • Restriction digests

Restriction Digest Overnights

Transformations

Cell competence protocols [[Media|Protocol_Quick_CaCl2_competence.pdf}}