Team:Brown/Protocols
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Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]]. | Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]]. | ||
- | + | *PCR | |
- | + | [[:Team:Brown/Protocols/PCR|PCR]] | |
- | + | *Ligations | |
- | + | [[:Team:Brown/Protocols/Ligation|Ligation]] | |
- | + | *Restriction digests | |
- | + | [[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest]] | |
- | + | Overnights | |
- | + | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | |
- | + | Transformations | |
- | + | *[[:Team:Brown/Protocols/Transformation|Transformation]] | |
- | + | Cell competence protocols | |
+ | [[Media|Protocol_Quick_CaCl2_competence.pdf}} |
Revision as of 04:31, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
- PCR
- Ligations
- Restriction digests
Restriction Digest Overnights
Transformations
Cell competence protocols [[Media|Protocol_Quick_CaCl2_competence.pdf}}