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Team:Harvard/color/parts
From 2010.igem.org
(Difference between revisions)
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<h2>RNAi constructs</h2> | <h2>RNAi constructs</h2> | ||
<table cellspacing="0"> | <table cellspacing="0"> | ||
| - | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr> | + | <tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr> |
<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr> | <tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr> | ||
<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr> | <tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr> | ||
Latest revision as of 00:25, 27 October 2010
abstract lab notebook pigment metabolism engineering pathways targeting the petals parts & primers results references
parts and primers
RNAi constructs
| Biobrick Name | Description | Size | Sequence |
| BBa_K382010 | amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown | 413 | link |
| BBa_K382011 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | 413 | link |
| BBa_K382012 | amiRNA construct for lycopene beta cyclase (LYC) knockdown | 413 | link |
Primers
| Name | Description | Sequence |
| CP1 | LUT2 I | gaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc |
| CP2 | LUT2 II | gaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga |
| CP3 | LUT2 III | gaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg |
| CP4 | LUT2 IV | gaAAACTTCGTAGTAACCCGTATtctacatatatattcct |
| CP13 | BETA-OHASE I | gaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc |
| CP14 | BETA-OHASE II | gaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga |
| CP15 | BETA-OHASE III | gaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg |
| CP16 | BETA-OHASE IV | gaACGTTTATATCGAAGAGATGCtctacatatatattcct |
| CP9 | LYC I | gaTAATTATCCAACGACTTGCAGtctctcttttgtattcc |
| CP10 | LYC II | gaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga |
| CP11 | LYC III | gaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg |
| CP12 | LYC IV | gaAAATTATGCAACGACTTGTAGtctacatatatattcct |
