Team:SDU-Denmark/project-i

From 2010.igem.org

(Difference between revisions)

Revision as of 21:31, 1 July 2010

The Idea

Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [http://microfluids.engin.brown.edu/Breuer_Papers/Journals/Small2008_Bacterial_Pump.pdf] we have decided to attempt construction of a similar system. Our approach centers on E. coli that will be modified to hyper-express flagella, in an attempt to increase force generation. These bacteria will be attached to a surface, and their flagella should therefor generate flow in the surrounding liquid.

Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.

Both sub-project will contribute new parts to the registry.

1. Microfluidic pump powered by self-organizing bacteria., Min Jun Kim and Kenneth S. Breuer; Small. 2008 January; 4(1): 111–118. doi: 10.1002/smll.200700641.

2. Photostimulation of a Sensory Rhodopsin II/HtrII/Tsr Fusion Chimera Activates CheA-Autophosphorylation and CheY-Phosphotransfer in Vitro†, Vishwa D. Trivedi and, John L. Spudich; Biochemistry 2003 42 (47), 13887-13892

--CKurtzhals 21:31, 1 July 2010 (UTC)

@@ Line 6: / Line 6: @@
 == The Idea ==
-Write content here.
+Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [http://microfluids.engin.brown.edu/Breuer_Papers/Journals/Small2008_Bacterial_Pump.pdf]
-</div>
+we have decided to attempt construction of a similar system. Our approach centers on E. coli that will be modified to hyper-express flagella, in an attempt to increase force generation. These bacteria will be attached to a surface, and their flagella should therefor generate flow in the surrounding liquid.
+Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [http://pubs.acs.org/doi/abs/10.1021/bi034399q], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.
+Both sub-project will contribute new parts to the registry.
 <div id="rightcolumn">
-Include column content here.
-</div>
+. ''Microfluidic pump powered by self-organizing bacteria.'', Min Jun Kim and Kenneth S. Breuer; Small. 2008 January; 4(1): 111–118. doi: 10.1002/smll.200700641.
+. ''Photostimulation of a Sensory Rhodopsin II/HtrII/Tsr Fusion Chimera Activates CheA-Autophosphorylation and CheY-Phosphotransfer in Vitro†'', Vishwa D. Trivedi and, John L. Spudich; Biochemistry 2003 42 (47), 13887-13892
 </div>
+--[[User:CKurtzhals|CKurtzhals]] 21:31, 1 July 2010 (UTC)