Team:Lethbridge/Results
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The placement of an oligoarginine sequence at the N-terminus of a protein will destabilize the protein in vivo. | The placement of an oligoarginine sequence at the N-terminus of a protein will destabilize the protein in vivo. | ||
==<b><font color="white">Introduction</font></b>== | ==<b><font color="white">Introduction</font></b>== | ||
- | The long term goal of our team is to utilize an oligoarginine tail to specifically target enzymes into a microcompartment composed of modified lumazine synthase subunits. While conducting research on the project, we came upon data originally reported by Bachmair <i>et al.</i><sup>1</sup> suggesting that | + | The long term goal of our team is to utilize an oligoarginine tail to specifically target enzymes into a microcompartment composed of modified lumazine synthase subunits. While conducting background research on the project, we came upon data originally reported by Bachmair <i>et al.</i><sup>1</sup> suggesting that the identity of the amino acid at the N-terminus of a protein is related to its half-life, and mostly notably, that arginine residues at the are destabilizing. This data suggests that by placing an arginine at the N-terminus of a protein to be targeted into a lumazine synthase microcompartment would cause degradation of our protein before it can be moved into the microcompartment. <br> |
We chose to investigate the how the placement of an oligoarginine sequence affects the stability of the protein to which it is fused. | We chose to investigate the how the placement of an oligoarginine sequence affects the stability of the protein to which it is fused. | ||