Team:MIT phage construction
From 2010.igem.org
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- | <br><br><b>BBa_K415147</b> | + | <br><br><b>BBa_K415147 - K415152</b> |
<ul><li> | <ul><li> | ||
- | Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII- | + | Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Zipper/li> |
- | <li>Explanation: | + | <li>Explanation: These parts are K415010 + RBS + P8-fusion protein. As is, this part produces mCherry and the p8-fusion protein in the presence of c6-AHL (and there's also non-trivial leaky expression). When combined with the Hyperphage plasmid, the p8-fusion protein should become incorporated into the phage coat along with normal p8, along for the zippers to be exposed on the polyphage hairs outside of the cell, allowing for potential cross-linking among cells. With the Collins toggle, UV exposure can be used to control the expression of the p8-fusion protein. |
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Revision as of 23:33, 24 October 2010
hairy cells and polymerizing phage - construction |
PARTS BBa_K415100
![]() BBa_K415108
![]() BBa_K415138
![]() BBa_K415147 - K415152
|