Team:Heidelberg/Notebook/Material Methods
From 2010.igem.org
(→Enzymes) |
(→Enzymes) |
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- | |+ align="top, left"|'''table 3''': Used | + | |+ align="top, left"|'''table 3''': Used NEB restriction enzymes |
| Name||Number/ID||NEB buffer1||NEB buffer2||NEB buffer3||NEB buffer4||BSA | | Name||Number/ID||NEB buffer1||NEB buffer2||NEB buffer3||NEB buffer4||BSA | ||
|- | |- | ||
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|BglI||-||50%||75%||100%||50%|| | |BglI||-||50%||75%||100%||50%|| | ||
|- | |- | ||
- | |BglII||13|| 10%|| 75%|| 100%|| 10% | + | |BglII||13|| 10%|| 75%|| 100%|| 10%|| |
|- | |- | ||
- | |BsrGI||-|| 25%|| 100%|| 10%|| 100% | + | |BsrGI||-|| 25%|| 100%|| 10%|| 100%||x |
|- | |- | ||
|DpnI ||6|| 100%|| 100%|| 75%|| 100%|| | |DpnI ||6|| 100%|| 100%|| 75%|| 100%|| | ||
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|SfcI ||19|| 75%|| 50%|| 10%|| 100%|| x | |SfcI ||19|| 75%|| 50%|| 10%|| 100%|| x | ||
|- | |- | ||
- | |SfcI||-|| | + | |SfcI||-||75%|| 50%|| 10%|| 100%|| x |
|- | |- | ||
|SpeI ||-|| 75%|| 100%|| 25%|| 100%|| x | |SpeI ||-|| 75%|| 100%|| 25%|| 100%|| x |
Revision as of 18:41, 24 October 2010
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