Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test30settembre

From 2010.igem.org

(Difference between revisions)
(→RESULTS)
 
Line 29: Line 29:
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
Line 42: Line 42:
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
-
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
+
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
==RESULTS==
==RESULTS==
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3491
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3491
|-
|-
-
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.3420
+
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.3420
|-
|-
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0584
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0584
Line 86: Line 86:
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
|-
|-
-
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> || -
+
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
|}
|}

Latest revision as of 21:28, 25 October 2010
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SEPTEMBER, 30TH


PLATE


UNIPV Pavia piastra30settembre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC3009.png
UNIPV Pavia FluoVSODCo3009.png
UNIPV Pavia FluoVSODLC03009.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.0308
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0.1484
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.4214
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> 0.3491
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.3420
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.0584
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.1452
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1177
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> 0.5602
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> 0.3985
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> 0.2414
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> -



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