Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1ottobre

From 2010.igem.org

OCTOBER, 1ST

PLATE

EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
<partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

The ODstart are reported in the table below:

Part	ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo>	0.0260
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo>	0.1024
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo>	0.3933
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo>	0.3466
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo>	0.3213
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo>	0.0522
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo>	0.1655
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo>	0.1058
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo>	0.5087
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo>	0.3559
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo>	0.2407
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo>	-

Tecan Test