From 2010.igem.org
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| | The ODstart are reported in the table below: | | The ODstart are reported in the table below: |
Revision as of 17:29, 24 October 2010
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SEPTEMBER, 30TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
Methods
The experiment was performed in M9 according to Protocol 1 on the following measurement parts:
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
- <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
RESULTS
Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
The ODstart are reported in the table below:
| Part | ODstart
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> | 0.0308
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> | 0.1484
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> | 0.4214
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> | 0.3491
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> | 0.3420
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> | 0.0584
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> | 0.1452
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> | 0.1177
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> | 0.5602
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> | 0.3985
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> | 0.2414
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> | -
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