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Team:Heidelberg/Notebook/miRNA Kit
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= Synthetic miRNA Kit = | = Synthetic miRNA Kit = | ||
| - | This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create | + | This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNA-like miRNAs and referring binding sites using an oligo and PCR based system. |
| - | You do not know where to start? Why | + | You do not know where to start? Why don't you [https://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner design your binding sites] first! |
| - | After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [https://2010.igem.org/Team:Heidelberg/Notebook/ | + | After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure mi Measure notebook] and see miMeasure in action on our [https://2010.igem.org/Team:Heidelberg/Project/miMeasure measurements] page! |
| - | Now you would like to deliver you vector to a patient | + | Now you would like to deliver you vector to a patient? Why not use a very specific [https://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling/ViroBytes shuttle]? |
Revision as of 14:03, 25 October 2010
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