Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
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Seed sequence (1-8) </span></div>
Seed sequence (1-8) </span></div>
<div class="option">
<div class="option">
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<SELECT name="seed" onclick="chMd1()" disabled="disabled">
+
<SELECT name="seed" onchange="chMd1()" disabled="disabled">
<OPTION value="6mer">6mer                (2-7 paired)
<OPTION value="6mer">6mer                (2-7 paired)
<OPTION value="7merA1">7merA1          (1-7 paired)
<OPTION value="7merA1">7merA1          (1-7 paired)
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</div>
</div>
<div class="option">
<div class="option">
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<SELECT name="comp" onclick="chMd2()" disabled="disabled">
+
<SELECT name="comp" onchange="chMd2()" disabled="disabled">
<OPTION value="3">3                  (14-16 paired)
<OPTION value="3">3                  (14-16 paired)
<OPTION value="4">4                  (13-16 paired)
<OPTION value="4">4                  (13-16 paired)
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<br>
<br>
<div class="option"><span style="font-size: 18.0px; text-decoration: underline;">Sticky ends for integration in plasmid: </span></div>
<div class="option"><span style="font-size: 18.0px; text-decoration: underline;">Sticky ends for integration in plasmid: </span></div>
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<div class="option">  <SELECT id="stickyends" name="stickyends" onclick="chMd3()">
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<div class="option">  <SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<OPTION SELECTED value="none">None
<OPTION SELECTED value="none">None
<OPTION value="BBB">Biobricks Standard RFC-12
<OPTION value="BBB">Biobricks Standard RFC-12
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</div>
</div>
<center>
<center>
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<input value="      Get your Binding Site!      " type="button" onClick="SBS(seqIn)">
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<input value="      Get your Binding Site!      " type="button" onclick="SBS(seqIn)">
</center>
</center>
<br>
<br>
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</div>
</div>
</body>
</body>
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<script type="text/javascript" src="Final_code_miBS_HD2010.txt"></script>
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<script type="text/javascript" src="https://static.igem.org/mediawiki/2010/2/24/Final_code_miBS_HD2010.txt"></script>
</html>
</html>
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{{:Team:Heidelberg/Pagemiddle}}
{{:Team:Heidelberg/Bottom}}
{{:Team:Heidelberg/Bottom}}

Revision as of 16:34, 24 October 2010

BS designer for miRNA


Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'


Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

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