Revision as of 11:57, 24 October 2010

Homology Based Capsid Shuffling

we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells.
cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
- AAV1 A
- AAV2 A & B
- AAV5 A & B
- AAV6
- AAV8 A & B
- AAV9 A

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 {{:Team:Heidelberg/Pagetop|home}}
 = Homology Based Capsid Shuffling =
+==31/08/2010==
+*we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells. <br />
+*cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
+*PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
+**AAV1 A
+**AAV2 A & B
+**AAV5 A & B
+**AAV6
+**AAV8 A & B
+**AAV9 A
 {{:Team:Heidelberg/Pagemiddle}}