Team:Heidelberg/Notebook
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== [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling Capsid shuffling] == | == [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling Capsid shuffling] == | ||
Did you ever get bored by the common viruses that infect randomly, irrational and uncontrolable? Never again! We created a library of synthetic viruses that can be evolved on any cell type of choice and enable you to '''select for your personal favourite virus, custom made!''' | Did you ever get bored by the common viruses that infect randomly, irrational and uncontrolable? Never again! We created a library of synthetic viruses that can be evolved on any cell type of choice and enable you to '''select for your personal favourite virus, custom made!''' | ||
- | === [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling/Homology_Based Homology Based] === | + | : === [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling/Homology_Based Homology Based] === |
This clever approach uses DNAseI shuffeling of AAV capsid genes followed by PCR amplification with primers for homologous regions. Find out how we mastered this approach! | This clever approach uses DNAseI shuffeling of AAV capsid genes followed by PCR amplification with primers for homologous regions. Find out how we mastered this approach! | ||
- | === [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling/ViroBytes ViroBytes] === | + | : === [https://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling/ViroBytes ViroBytes] === |
Last year's Alberta BioBytes served as the basis for our '''ViroBytes''' method. We optimized the protocol for production of '''rationally shuffled synthetic viruses'''. | Last year's Alberta BioBytes served as the basis for our '''ViroBytes''' method. We optimized the protocol for production of '''rationally shuffled synthetic viruses'''. | ||
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== [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure miMeasure] == | == [https://2010.igem.org/Team:Heidelberg/Notebook/miMeasure miMeasure] == | ||
'''THE''' standard for characterization of synthetic miRNA binding sites in mammalian cells! | '''THE''' standard for characterization of synthetic miRNA binding sites in mammalian cells! |
Revision as of 11:33, 24 October 2010
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