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Team:Imperial College London/Chassis
From 2010.igem.org
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[[Image:IC_2010_IGEMchassis.jpg|thumb|center|800px]] | [[Image:IC_2010_IGEMchassis.jpg|thumb|center|800px]] | ||
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| + | E.coli was considered as a possible option. Despite the gram negative outer membrane, there are strains that have been made more permeable through knockout of Lipid A biosynthesis in lipopolysaccharides [4] There are also proteins that can disrupt membranes when they are inserted in them, thus making them more permeable. These chages in permeability are likely due to transient ruptures of outer membrane and so unlikely to make a very responsive or robust detecton organism [4]. | ||
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{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:25px;" cellspacing="20" | {| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:25px;" cellspacing="20" | ||
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Surplus information on B. Subtilis chassis [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Main_Page Subti-Wiki] | Surplus information on B. Subtilis chassis [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Main_Page Subti-Wiki] | ||
| - | + | [http://mic.sgmjournals.org/cgi/content/full/146/12/3025 1] | |
| - | + | [http://www.microbialcellfactories.com/content/5/1/22 2] | |
| - | + | [http://www3.interscience.wiley.com/cgi-bin/fulltext/118603263/PDFSTART 3] | |
| - | + | [http://aac.asm.org/cgi/content/full/43/6/1459?view=long&pmid=10348770 4] | |
| - | + | ||
|} | |} | ||
Revision as of 08:28, 24 October 2010
| Chassis |
|
Our choice of chassis was B. subtilis.  |
| Bacillus | Breakdown |
 E.coli was considered as a possible option. Despite the gram negative outer membrane, there are strains that have been made more permeable through knockout of Lipid A biosynthesis in lipopolysaccharides [4] There are also proteins that can disrupt membranes when they are inserted in them, thus making them more permeable. These chages in permeability are likely due to transient ruptures of outer membrane and so unlikely to make a very responsive or robust detecton organism [4]. |
| References |
|
Surplus information on B. Subtilis chassis [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Main_Page Subti-Wiki] [http://mic.sgmjournals.org/cgi/content/full/146/12/3025 1] [http://www.microbialcellfactories.com/content/5/1/22 2] [http://www3.interscience.wiley.com/cgi-bin/fulltext/118603263/PDFSTART 3] [http://aac.asm.org/cgi/content/full/43/6/1459?view=long&pmid=10348770 4] |
