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Team:St Andrews/project/laboratory/protocols
From 2010.igem.org
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===Transformation=== | ===Transformation=== | ||
| - | NEB Turbo Competent E. coli | + | [http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli] |
| - | Clontech Stellar Competent E. coli. | + | [http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.] |
===Restriction Digest=== | ===Restriction Digest=== | ||
Revision as of 15:18, 23 October 2010
Contents |
Protocols
We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.
Cell Culture
OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]
Transformation
[http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli]
[http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.]
Restriction Digest
Promega Enzymes
NEB Biobrick Assembly Kit (Gingko Bioworks)
Ligation
NEB Biobrick Assembly Kit (Gingko Bioworks)
PCR
OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]
NEB Taq
Finnzymes Phusion
DNA Extraction
Quiagen Miniprep Isopropanol Extraction of small DNA fragments
Construction of BioBricks
Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)
OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)
Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)
OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]
