Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
Line 30: Line 30:
Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
</td>  
</td>  
-
<td>744 bp</td><td></td>
+
<td>744 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 47: Line 47:
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
</td>  
</td>  
-
<td>253 bp</td><td></td>
+
<td>253 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 66: Line 66:
Seq ok(MIT)
Seq ok(MIT)
</td>  
</td>  
-
<td>403 bp</td><td></td>
+
<td>403 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 84: Line 84:
Seq ok(MIT 2009); length ok
Seq ok(MIT 2009); length ok
</td>  
</td>  
-
<td>769 bp</td><td></td>
+
<td>769 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 101: Line 101:
Seq ok(MIT)
Seq ok(MIT)
</td>  
</td>  
-
<td>406 bp</td><td></td>
+
<td>406 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 119: Line 119:
Seq ok(MIT); length ok
Seq ok(MIT); length ok
</td>  
</td>  
-
<td>664 bp</td><td></td>
+
<td>664 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 136: Line 136:
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
</td>  
</td>  
-
<td>718 bp</td><td></td>
+
<td>718 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
Line 154: Line 154:
Wrong clone
Wrong clone
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 172: Line 172:
Seq ok(BMR); length ok
Seq ok(BMR); length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 189: Line 189:
not sequenced, length ok
not sequenced, length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 206: Line 206:
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 225: Line 225:
Seq ok(BMR); Cm resistance ok; length ok.
Seq ok(BMR); Cm resistance ok; length ok.
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 243: Line 243:
not sequenced; Cm resistance ok
not sequenced; Cm resistance ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 261: Line 261:
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 278: Line 278:
Wrong clone
Wrong clone
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 295: Line 295:
not sequenced; length ok
not sequenced; length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 312: Line 312:
Seq ok(BMR); length ok
Seq ok(BMR); length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 329: Line 329:
Seq ok(BMR); Cm resistance ok; length ok
Seq ok(BMR); Cm resistance ok; length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 346: Line 346:
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 363: Line 363:
not sequenced
not sequenced
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
Line 380: Line 380:
none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>BioBricks 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>BioBricks 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
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none
none
</td>  
</td>  
-
<td></td><td></td>
+
<td></td><td>Ligations 2010</td>
</tr>
</tr>
</table>
</table>

Revision as of 11:15, 25 June 2010
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Freezer Management



Biobrick or
ligation name		DescriptionCreation dateStrainVectorQuality controlLengthStore

<partinfo>BBa_E2050</partinfo>

mOrange

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

744 bpBioBricks 2010

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

253 bpBioBricks 2010

<partinfo>BBa_K165037</partinfo>

tef2 promoter

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(MIT)

403 bpBioBricks 2010

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok(MIT 2009); length ok

769 bpBioBricks 2010

<partinfo>BBa_J61001</partinfo>

R6K origin

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT)

406 bpBioBricks 2010

<partinfo>BBa_K081008</partinfo>

RBS-luxI

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT); length ok

664 bpBioBricks 2010

<partinfo>BBa_K125500</partinfo>

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

I0-1

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

Ligations 2010

I0-2

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

Ligations 2010

I1-1
(not used)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

not sequenced, length ok

Ligations 2010

I1-2

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok

Ligations 2010

I2-1

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); Cm resistance ok; length ok.

Ligations 2010

I2-2
(not used)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; Cm resistance ok

Ligations 2010

I3-1

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok

Ligations 2010

I3-2

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

Ligations 2010

I4-1
(not used)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; length ok

Ligations 2010

I4-2

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

Ligations 2010

I5

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(BMR); Cm resistance ok; length ok

Ligations 2010

I6-2

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok

Ligations 2010

I6-3
(not used)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

not sequenced

Ligations 2010

<partinfo>BBa_K208001</partinfo> (stab-1)

 phasin phaP

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

none

BioBricks 2010

<partinfo>BBa_K208001</partinfo> (stab-2)

 phasin phaP

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

none

BioBricks 2010

I7-1

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I7-2

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I8-1

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I8-2

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I9-1

 <partinfo>BBa_J23111</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I9-2

 <partinfo>BBa_J23111</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I10-1

 <partinfo>BBa_J23116</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010

I10-2

 <partinfo>BBa_J23116</partinfo> (E-S)
+
I6-2 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

none

Ligations 2010
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