Team:TU Delft/Project/sensing/characterization

(Difference between revisions)

Revision as of 13:21, 25 October 2010

Strains:

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 Strains:
-* PalkS / AlkS: E.coli K12/310C
+* P(CaiF)-E0240: E.coli K12/331A
-* PalkS / AlkS / PalkB: E.coli K12/311C
+* Negative control: ''E.coli K12'' carrying J13002 on the empty plasmid pSB1A2.
-* P(CaiF) / AlkS / PalkB: E.coli K12/312C
-* Negative control: ''E.coli K12''
-The characterization process of the hydrocarbon sensing sub-project will entail output measurements of fluorescence at varying octane concentrations (0.1% – 1%) of the above mentioned strains. These measurements will be performed using a 96-well plate reader. Growth curves will be followed by OD-600 alongside the fluorescence measurements. The obtained fluorescence and growth measurements will be analyzed and modeled accordingly.
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