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Korea U Seoul/2 October 2010
From 2010.igem.org
(Difference between revisions)
(New page: ===Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3=== <BR> {| style="border: 1px solid red;" |Reagent |Volume (μL) |- |10x T4 DNA ligase reaction buffer |2 |- |T4 DN...) |
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===Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3=== | ===Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3=== | ||
<BR> | <BR> | ||
| - | {| style="border: 1px solid | + | {| style="border: 1px solid black;" |
| - | |Reagent | + | |'''Reagent''' |
| - | |Volume (μL) | + | |'''Volume (μL)''' |
|- | |- | ||
|10x T4 DNA ligase reaction buffer | |10x T4 DNA ligase reaction buffer | ||
| Line 17: | Line 17: | ||
|8 / total = 20 | |8 / total = 20 | ||
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| - | !COLSPAN="2"| Incubation at room temperature for 30min | + | ! COLSPAN="2"| Incubation at room temperature for 30min<br>and stop at 80˚C for 20min |
| - | <br>and stop at 80˚C for 20min | + | |
|} | |} | ||
Latest revision as of 13:46, 20 October 2010
Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3
| Reagent | Volume (μL) |
| 10x T4 DNA ligase reaction buffer | 2 |
| T4 DNA ligase | 2 |
| Each of the digests | 2 + 2 + 2 = 8 |
| D.W. | 8 / total = 20 |
| Incubation at room temperature for 30min and stop at 80˚C for 20min | |
|---|---|
