Team:Imperial College London/Software Tool
From 2010.igem.org
(Difference between revisions)
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det[5] = "The complement system is part of the innate immune response to an acute infection. Detecting C3 convertase could give medical professionals a rapid method of seeing if a patient is fighting an acute infection."; | det[5] = "The complement system is part of the innate immune response to an acute infection. Detecting C3 convertase could give medical professionals a rapid method of seeing if a patient is fighting an acute infection."; | ||
var prefix = "GAATTCGCGGCCGCTTCTAG"; | var prefix = "GAATTCGCGGCCGCTTCTAG"; | ||
- | var promoter = " | + | var promoter = "AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTTGTACAATAAATGT"; |
var rbs = "TACTAGAGTCTTCAGAAAGGAGT"; | var rbs = "TACTAGAGTCTTCAGAAAGGAGT"; | ||
- | var scar = " | + | var scar = "TACTAG"; |
- | var cwb = " | + | var cwb = "ATGCGTTCTTATATAAAAGTCCTAACAATGTGTTTTCTGGGGCTCATACTTTTTGTGCCAACAGCTTTGGCCGATAACTCAGTGAAAAGAGTTGGGGGAAGCAATAGATACGGCACTGCTGTACAAATATCAAAGCAAATGTATTCAACAGCAAGTACAGCTGTAATTGTTGGTGGGAGTTCCTATGCAGATGCTATTTCAGCAGCACCTCTTGCTTACCAGAAGAATGCGCCATTGCTTTACACTAATTCTGATAAGCTTTCATATGAAACGAAAACAAGATTGAAAGAGATGCAGACTAAAAATGTAATTATTGTAGGCGGAACACCTGCTGTTTCTTCTAACACTGCTAACCAGATTAAAAGCTTGGGGATAAGTATTAAACGAATTGCAGGAAGCAACCGTTATGATACGGCTGCACGGGTGGCAAAAGCGATGGGTGCGACTTCAAAAGCTGTTATTTTGAACGGCTTCTTATATGCAGACGCTCCGGCCGTCATCCCTTATGCAGCGAAAAACGGGTATCCAATTCTTTTTACAAATAAAACATCTATAAATAGTGCGACTACGTCTGTGATAAAAGATAAGGGAATTTCGAGTACCGTTGTTGTAGGAGGCACTGGAAGTATCAGCAATACGGTATACAACAAGTTACCTTCTCCTACAAGAATTAGCGGTTCAAACAGATATGAGCTTGCTGCAAATATCGTACAAAAACTTAATTTATCAACAAGCACCGTATATGTAAGCAATGGATTCAGCTACCCTGACTCTATTGCAGGAGCTACACTGGCAGCTAAGAAGAAGCAATCTCTTATTCTTACAAATGGTGAAAATTTATCTACAGGAGCCCGTAAAATTATTGGAAGTAAAAACATGTCAAACTTTATGATTATCGGAAACACTCCTGCCGTAAGCACAAAGGTTGCTAATCAGCTAAAGAATCCAGTTGTA"; |
- | var linker = " | + | var linker = "AGCAGGGGTAGCAGGGCTGGTGGCGGTGGCGGTGGC"; |
var cleavage = new Array(); | var cleavage = new Array(); | ||
cleavage[1] = "AGCTGGCCTCTT"; | cleavage[1] = "AGCTGGCCTCTT"; | ||
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cleavage[4] = "ATCGACGGACGT"; | cleavage[4] = "ATCGACGGACGT"; | ||
cleavage[5] = "TTATTATCTCGTTCTGAAGAAGAC"; | cleavage[5] = "TTATTATCTCGTTCTGAAGAAGAC"; | ||
- | var aip = " | + | var aip = "GAAATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTTCAAAGGAAAAAA"; |
var terminator = "TAATAA"; | var terminator = "TAATAA"; | ||
var suffix = "TACTAGTAGCGGCCGCTGCAG"; | var suffix = "TACTAGTAGCGGCCGCTGCAG"; | ||
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|- | |- | ||
|style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html> | |style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html> | ||
- | <div id="sequenceout"><span id="stem">Awaiting sequence generation... </span><span id="spre" style="color:#F6CF39"></span><span id="spro" style="color:#FC8E42"></span><span id="srbs" style="color:#D34649"></span><span id="ssca" style="color:# | + | <style type="text/css"> |
+ | #sequenceout { | ||
+ | width:500px;} | ||
+ | </style> | ||
+ | <div id="sequenceout"><span id="stem">Awaiting sequence generation... </span><span id="spre" style="color:#F6CF39"></span><span id="spro" style="color:#FC8E42"></span><span id="srbs" style="color:#D34649"></span><span id="ssca" style="color:#A851AA"></span><span id="scwb" style="color:#AB6197"></span><span id="slin" style="color:#6273A8"></span><span id="scle" style="color:#6A9ECD"></span><span id="saip" style="color:#68904E"></span><span id="ster" style="color:#9DB742"></span><span id="ssuf" style="color:#F6CF39"></span></div> | ||
</html>''' | </html>''' | ||
|- | |- | ||
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'''<span style="color:#D34649">Red</span> - Ribosome Binding Site''' | '''<span style="color:#D34649">Red</span> - Ribosome Binding Site''' | ||
- | '''<span style="color:# | + | '''<span style="color:#A851AA">Violet</span> - Scar''' |
'''<span style="color:#AB6197">Purple</span> - Cell Wall Binding Domain''' | '''<span style="color:#AB6197">Purple</span> - Cell Wall Binding Domain''' |
Revision as of 14:13, 20 October 2010
Software Tool |
We realised early on that our detection module could be designed with a sensitivity to different proteases. By changing the cleavage site the system can accept a wide variety of inputs. This tool is designed to facilitate a quick custom sequence generation of the entire surface protein construct. |
Select Protease | Description | |
This was our primary target. Read our wiki to find out more! | ||
Awaiting sequence generation...
| ||
Yellow - Biobrick Prefix/Suffix Orange - Promoter Red - Ribosome Binding Site Violet - Scar Purple - Cell Wall Binding Domain Dark Blue - Adjustable Linker Light Blue - Protease Cleavage Site Dark Green - Autoinducing Peptide Light Green - Terminator |