From 2010.igem.org
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| | <h1>Protocols</h1> | | <h1>Protocols</h1> |
| | In this section we will offer protocols for techniques we have used that are not part of comerically avalible kits. | | In this section we will offer protocols for techniques we have used that are not part of comerically avalible kits. |
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| | <h2>Ligase Free Cloning</h2> | | <h2>Ligase Free Cloning</h2> |
| | This technique | | This technique |
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| - | <b>Metabolic Engineering: In vivo Nanobioreactors</b> | + | <b>Step 1</b> |
| | + | Information |
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| - | Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment. Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes. To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.
| + | <b>Step 2</b> |
| | + | Information. |
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| - | <b>Athina Software Package</b> | + | <h2>Protocol 2</h2> |
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| - | <b>A</b>utomated <b>Th</b>eoretical <b>I</b>nteraction <b>N</b>etwork <b>A</b>ssembly is a web service to transform a sequence of BioBricks, or any other set of biomolecular components, to a set of reactions that can be simulated dynamically. The user simply inputs the Biobricks and their relationships and Designer builds a reaction network using biological interactions rules. This software package also includes a wiki. This is a web service to collect the kinetic parameters necessary to create a model that can be simulated. | + | <b>Step 1</b> |
| | + | Information |
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| - | <h2>Highlights for the Judges</h2> | + | <b>Step 2</b> |
| - | Judges! Please navigate to our [https://2010.igem.org/Team:Minnesota/Criteria Judging Criteria] page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.
| + | Information. |
Revision as of 17:59, 17 October 2010
Protocols
In this section we will offer protocols for techniques we have used that are not part of comerically avalible kits.
Ligase Free Cloning
This technique
Step 1
Information
Step 2
Information.
Protocol 2
about
Step 1
Information
Step 2
Information.
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