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Team:WITS-South Africa/Machine Design
From 2010.igem.org
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| + | == Machine Construction == | ||
| + | Standard assembly etc etc | ||
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| + | == Machines Constructed== | ||
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| + | === Final Machines === | ||
| + | ==== Machine 1 ==== | ||
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| + | Final Machine 1 (Figure 2) | ||
| + | Purpose: | ||
| + | a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal | ||
| + | [[Image: machine_1_small.jpg|650px]] | ||
| + | [[Image:Venus-tt_screen.jpg|350px]] | ||
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| + | ==== Machine 2 ==== | ||
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| + | |||
| + | === Intermediate Machines === | ||
Intermediate Machine 2 (Figure 1) | Intermediate Machine 2 (Figure 1) | ||
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Fig1. The proposed intermediate Machine 2 | Fig1. The proposed intermediate Machine 2 | ||
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Fig 2. The proposed Final Machine 1 | Fig 2. The proposed Final Machine 1 | ||
Revision as of 19:17, 17 October 2010
Contents |
Machine Construction
Standard assembly etc etc
Machines Constructed
Final Machines
Machine 1
Final Machine 1 (Figure 2)
Purpose:
a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal
Machine 2
Intermediate Machines
Intermediate Machine 2 (Figure 1) Purpose: a) To characterise the strength of the induction of the LacI/AraC promoter by measuring fluorescence after IPTG is added b) To show that the PlcR promoter is activated in L. gasseri by measuring fluorescence after the addition of exogenous PlcR and PapR proteins
Fig1. The proposed intermediate Machine 2
Fig 2. The proposed Final Machine 1
Final Machine 2 (Figure 3) Purpose a) To act as the ‘Reporter’ Machine within the population and respond to infection as previously described
Fig 3. The proposed Final Machine 2
