Team:Imperial College London/Media

From 2010.igem.org

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Revision as of 12:05, 16 October 2010

Video Diary
Photos

Logos

Our Imperial College iGEM Logo
PARASIGHT
Another really cool logo

The Team

The team with advisors Chris and Tom
The Imperial College iGEM Team
Some team members with SynBio icon Jay Keasling!!!
Maddie and a bit of Kirill in labs
Maddie in labs again
Nick
Maddie in labs... AGAIN
Kirill, Maddie and Nick
Maddie
Maddie
Maddie
Nick (posing?)
Nick again
Piotr focusing, Anita not so much!
Piotr and Anita

Brainstorming

Fv-fragment guided input module
Ideas on parasite detection
Nick's idea on cell population control
Nick's idea...
Nick's idea...
Nick's idea...

Wet Lab

Surface Protein

Surface Protein Team Logo
Assembly Strategy for LytC
Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
Gel of PCR confirming pSB1C3 has been amplified.
Picture estimating the concentration of purified BOO14 and pSB1C3.
Colony PCR to detect sucessfully transformed bacteria.
3 PCR reactions to determine the most effective protocol to amplify the CWB.
Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
Isolation of the promoter pVEG by gel purification.
Determining the ratio of pSB1C3 to pVEG for ligation.
Determining the ratio of lytC to pSB1C3 for ligation.

Vectors

Vectors Team Logo
Assembly strategy for the AmyE vector
Assembly strategy for the PyrD vector
K14002 and oligo assembly
K143070 and K11064 assembly step

XylE

XylE Team Logo
Team XylE
The J23101 gene in a biobrick vector containg RFP gene.
Midi-prep XylE digestion with xbaI and PstI
Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
Catechol assay (before)
Catechol assay (after) - Can you spot the difference?
Catechol assay on XylE-trasformed cells in a 96-well plate
First Successful Catechol Assay!
First Successful Catechol Assay!
:)
Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
The coloured pigment is transported out of the cells.
Spectra of XylE transformed E.coli after addition of catechol assay
Catechol Assay cuvette setup
Catechol Assay cuvette setup

Strategy

The full assembly strategy
Wolf's primers

Parts

BioBricks
ComE binding sites in the promoters of the comCDE and comAB operons

Detection Module

Dr Martha Betson explaining the Schistosoma lifecycle

Fast Response Module

Results of a BLAST search using ComE (Accession no. AAC44897)
Results of a BLAST search using BlpR (Accession no. CAC03515.1)

Output Module

Human Practices

Human Practices panel: advisors
The team looks reeeeaaaalllly interested
Maddie's notes from the HP panel
Notes...
Notes...

School Workshops

Brainstorming
Chameleon: Now you see me - now you don't
More brainstorming
...and more brainstorming
Ideas on regulation of Synthetic Biology
H'n'H sauce (Hot&Healthy) - yummy
More ideas on regulations of SynBio
Brainstorming again
iGEM <3
Check out the lens creating bacteria!
More ideas...
Happy Bacteria?!
Regulations...
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