Team:Caltech/Protocols

From 2010.igem.org

(Difference between revisions)
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* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
* Making stock solutions
* Making stock solutions
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* Freezer (glycerol) stocks
+
* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
* Plating antibiotics
* Plating antibiotics
* Sequencing primers
* Sequencing primers

Revision as of 22:24, 19 October 2010


iGEM 2010



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  • Competent Cells
  • Transformation by Electroporation
  • Making stock solutions
  • Freezer (glycerol) stocks
  • Plating antibiotics
  • Sequencing primers
  • Making synthase casette
    • Sources of sequences
    • Optimizing & checking genes
  • Chemical/Crosslinking reactions
    • Digesting substrate (hydrolysis) + epoxidation (MCPBA)
    • crosslinking (AIBN3) + UV initiation
  • Inducing lysis via 632nm light initiation
  • Making cell/glycerol/agar mixture
    • Applying this to plates
  • Growing cells with PHA (and substrate oil)
  • Colony PCR
  • Two-day Cloning
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