Team:UPO-Sevilla/Notebook/08 06

From 2010.igem.org

(Difference between revisions)
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...)
 
(4 intermediate revisions not shown)
Line 19: Line 19:
       <h2>Production Team</h2>
       <h2>Production Team</h2>
-
       <p><strong>Paola Gallardo.</strong> Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p>
+
       <p>Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p>
-
      <p><strong>David Caballero.</strong> We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps are: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.</p>
 
       <h2>Assembly Team</h2>
       <h2>Assembly Team</h2>
-
       <p>Minpreps using inocula we put yesterday. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 & UPO11+2.</p>
+
      <p>We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps were: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.</p>
 +
 
 +
       <p>Minpreps using inocula we set up the day before. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 and UPO11+2.</p>
 +
 
 +
    <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_05" title="Go to 5th of August"><span>Go to 5th of August</span></a>
 +
 
 +
    <a class="next_button" href="/Team:UPO-Sevilla/Notebook/08_09" title="Go to 9th of August"><span>Go to 9th of August</span></a>
 +
 
 +
    <div class="clear"></div>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>

Latest revision as of 14:50, 27 October 2010

Home Banner

August, 6th

Production Team

Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.

Assembly Team

We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps were: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.

Minpreps using inocula we set up the day before. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 and UPO11+2.

Go to 5th of August Go to 9th of August
Return to Notebook

Footer

Sponsors

Retrieved from "http://2010.igem.org/Team:UPO-Sevilla/Notebook/08_06"