Team:IvyTech-South Bend/Notebook

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Latest revision as of 00:44, 28 October 2010

Discussion

Notebook

June
M	T	W	T	F	S	S
	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_June_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_June_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_June_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_June_2010&action=edit 4]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_June_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_June_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_June_2010&action=edit 8]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_June_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_June_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_June_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_June_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_June_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_June_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_June_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_June_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_June_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_June_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_June_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_June_2010&action=edit 22]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_June_2010&action=edit 23]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_June_2010&action=edit 24]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_June_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_June_2010&action=edit 26]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_June_2010&action=edit 27]
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_June_2010 28]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/29_June_2010 29]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_June_2010 30]

July
M	T	W	T	F	S	S
			[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_July_2010 1]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_July_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_July_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_July_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_July_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_July_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_July_2010&action=edit 8]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_July_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_July_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_July_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_July_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_July_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_July_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_July_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_July_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_July_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_July_2010&action=edit 20]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_July_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_July_2010&action=edit 22]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_July_2010&action=edit 23]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_July_2010&action=edit 24]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_July_2010&action=edit 26]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_July_2010&action=edit 27]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_July_2010 28]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_July_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_July_2010&action=edit 30]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_July_2010&action=edit 31]

August
M	T	W	T	F	S	S
						[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_August_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_August_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_August_2010&action=edit 4]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_August_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_August_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_August_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_August_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_August_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_August_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_August_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_August_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_August_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_August_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_August_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_August_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_August_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_August_2010&action=edit 20]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_August_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_August_2010&action=edit 23]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_August_2010 24]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_August_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_August_2010&action=edit 26]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/27_August_2010 27]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_August_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_August_2010&action=edit 30]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/31_August_2010 31]

September
M	T	W	T	F	S	S
		[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_September_2010 1]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/2_September_2010 2]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/3_September_2010 3]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_September_2010&action=edit 4]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_September_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_September_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_September_2010&action=edit 8]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/9_September_2010 9]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/10_September_2010 10]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_September_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_September_2010&action=edit 13]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_September_2010 14]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_September_2010 15]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/16_September_2010 16]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/17_September_2010 17]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_September_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_September_2010&action=edit 20]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_September_2010 21]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_September_2010 22]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_September_2010 23]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_September_2010 24]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_September_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_September_2010&action=edit 27]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_September_2010 28]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_September_2010&action=edit 29]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_September_2010 30]

October
M	T	W	T	F	S	S
				[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_October_2010 1]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_October_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_October_2010&action=edit 4]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/5_October_2010 5]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_October_2010 6]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/7_October_2010 7]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/8_October_2010 8]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_October_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_October_2010&action=edit 10]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_October_2010&action=edit 11]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/12_October_2010 12]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/13_October_2010 13]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_October_2010 14]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_October_2010 15]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_October_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_October_2010&action=edit 17]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_October_2010&action=edit 18]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/19_October_2010 19]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/20_October_2010 20]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_October_2010 21]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_October_2010 22]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_October_2010 23]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_October_2010 24]
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/25_October_2010 25]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/26_October_2010 26]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_October_2010&action=edit 27]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_October_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_October_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_October_2010&action=edit 30]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_October_2010&action=edit 31]

November
M	T	W	T	F	S	S
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_November_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_November_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_November_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_November_2010&action=edit 4]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_November_2010&action=edit 5]	[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_November_2010 6]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_November_2010&action=edit 7]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_November_2010&action=edit 8]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_November_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_November_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_November_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_November_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_November_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_November_2010&action=edit 14]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_November_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_November_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_November_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_November_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_November_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_November_2010&action=edit 20]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_November_2010&action=edit 21]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_November_2010&action=edit 22]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_November_2010&action=edit 23]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_November_2010&action=edit 24]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_November_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_November_2010&action=edit 26]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_November_2010&action=edit 27]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_November_2010&action=edit 28]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_November_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_November_2010&action=edit 30]

IGEM Part #s and Uses

BBa_T9002 – Producer Controler Device–Plate2 Well 9A

BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K

BBa_F2620– LuxR with terminator– Plate 2 Well 6E

BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A

BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O

BBa_pSB1C3—Plasmid—Plate 1 Well 3A

BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A

BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A

6/28/10

Preparing SOB

6/30/10

Richard Lab:Electroporation of E. coli

7/28/10

Usage and extraction

8/24/10

Today we will be pouring new plates for streaking new transformed cells.

8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated.

8/27/10

Today we will be electroplating part KBBa_3131010

8/31/10

Results of transformation - the plates had growth but (no) distinct colony pattern.

8/31/10

After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH

9/1/10

Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.

9/2/10

Results from streaking –

9/2/10

Protocol For Making LB-Broth/Agar

9/3/10

Today I’m finishing making the LB/Agar from yesterday.

9/9/10

Due to conflict we will be changing from E Coli to yeast.

9/10/10

Lux Casette Right Promoter BBa_I1051

9/14/10

Today we found growth from our Electroporated E – Coli parts

9/15/10

Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay

9/16/10

Today we will wash

9/17/10

Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!

10/18/10

cutting enzymes

9/21/10

Today we will electroporate Agrobacterium with part T9002

Electrophoresis

9/22/10

Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample

9/22/10

The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens

9/23/10

Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.

9/24/10

Today I’m testing absorbance of our trials @ 395nm

9/28/10

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10

9/30/10

LacZ without GFP also with RBS and terminator

10/1/10

electrophoresis

10/5/10

pTet GFP

10/7/10

Electroporating 2 parts with E-coli bacteria

10/8/10

LB-Lennox Broth & Electroporation

10/12/10

hold until Friday

10/13/10

Discoveries!

10/15/10

Using openwetware.org protocol

B-galactosidase Assay

BBL Trypticase soy Broth and Agar

DPBS

10/19/10

Protocol-- Electroporation man for E-Coli

10/21/10

High Efficiency Electrotransformation of E.coli

10/25/10

DNA from BlueGuy

10/26/10

Final steps pack and ship

Your team picture

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety

@@ Line 48: / Line 48: @@
 ==Notebook==
+<html>
+<style type="text/css">
+table.calendar          { margin: 0; padding: 10px; }
+table.calendar td       { margin: 0; padding: 2px; vertical-align: top; }
+table.month .heading td { padding:2px; background-color:#0f6666; color:#00ff00; text-align:center; font-size:120%; font-weight:bold; }
+table.month .dow td     { color:#00ff00; text-align:center; font-size:110%; }
+table.month td.today    { background-color:#ddd;}
+table.month td {
+    border: none;
+    margin: 0;
+    padding: 1.5pt 2pt;
+    font-weight: bold;
+    font-size: 10pt;
+    text-align: center;
+    background-color: #000000;
+    }
+#bodyContent table.month a { background:none; padding:0 }
+.day-active { color:#00FF00 }
+.day-empty  { color:#006666 }
+</style>
+</html>
+{| cellpadding="20" align="center"
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }}
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}
+|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }}
+|}
+===IGEM Part #s and Uses===
-{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }}
+BBa_T9002 – Producer Controler Device–Plate2 Well 9A
+BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
+BBa_F2620– LuxR with terminator– Plate 2 Well 6E
+BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
-{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }}
+BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
+BBa_pSB1C3—Plasmid—Plate 1 Well 3A
+BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
+BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
+== 6/28/10 ==
+Preparing SOB
+== 6/30/10 ==
+Richard Lab:Electroporation of E. coli
+== 7/28/10 ==
+Usage and extraction
 == 8/24/10 ==
 Today we will be pouring new plates for streaking new transformed cells.
@@ Line 62: / Line 113: @@
-	We will be running a gel to determine if our DNA sample was successfully electroplated.
+We will be running a gel to determine if our DNA sample was successfully electroplated.
 == 8/27/10 ==
+Today we will be electroplating part KBBa_3131010
-	Today we will be electroplating part KBBa_3131010
 == 8/31/10 ==
+Results of transformation - the plates had growth but (no) distinct colony pattern.
-	Results of transformation - the plates had growth but (no) distinct colony pattern.
 == 8/31/10 ==
+After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
-	After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
 == 9/1/10 ==
-	Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
+Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
 == 9/2/10 ==
 Results from streaking –
 == 9/2/10 ==
 Protocol For Making LB-Broth/Agar
 == 9/3/10 ==
 Today I’m finishing making the LB/Agar from yesterday.
 == 9/9/10 ==
 Due to conflict we will be changing from E Coli to yeast.
 == 9/10/10 ==
 Lux Casette Right Promoter BBa_I1051
@@ Line 112: / Line 153: @@
 == 9/14/10 ==
+Today we found growth from our Electroporated E – Coli parts
-	Today we found growth from our Electroporated E – Coli parts
 == 9/15/10 ==
+Today we will be extracting DNA from out electroporated cells/T9002
+B-galactosidase Assay
-	- Today we will be extracting DNA from out electroporated cells/T9002
+== 9/16/10 ==
+Today we will wash
+== 9/17/10 ==
-== 9/16/10 ==
+Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
-	Today we will wash our electrocomp grown t/pos bacteria with 1.2 mL Glycerol then electroporate
+== 10/18/10 ==
-Following protocol from page 15
-First prof. T sterilized the glycerol passing it through the .2 micron filter into 50 mL sterile centrifuge tube
-– then we added 1 mL 10 percent Glycerol to suspended cells
-– then centrifuged at 1000 rpm for 10 min
-- then removed supernate with BR 1000 micropipettor
-- then add 1 mL 10 percent Glycerol then repeat steps 1 & 2 “4” times then remove sup. and freeze cells at -80 degrees C freezer.
-- Today we also set up a new IGem work are in back lab look at pic
+cutting enzymes
+== 9/21/10 ==
-== 9/17/10 ==
+Today we will electroporate  Agrobacterium with part T9002
+Electrophoresis
-	Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast
+== 9/22/10 ==
-this will also Work!
-Following protocols from pgs 15 &19
+Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
--instead of Electroporating our part we will use PG10 to determine if these lines are good hosts
-First we will be pouring a full pack of LB/Agar/Amp plates using the LB/Agar I made on 9/2/10 then adding amp made by DG on 9/1/10 we will also add 5 mL Arabonse to the Agar before pouring plates have been poured now letting stand for an hour to harden.
+== 9/22/10 ==
--Now I will be making 1L of LB/Broth for Culturing.
+The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
-Lot 9082989 ex. 2014-02-28
-BD Difco LB/Broth – Lennox 20 g per 1L
-so I will put 10 g into 2 elenmyier flask/capped one weighed 10.0023 g and the other weighed 10.0011 g then I added 500 mL into each flask then shook to mix then dissolved in microwaved for 8 oz setting once then placed into Autoclave for 15 min @ 121 degrees C until done.
-Now we are going electroporate our grown t/pos Electro Comp Cells from yesterday with PG10 using protocol from pg 15 changing # 5 to 5 mL of PG10
+== 9/23/10 ==
-We will be using a different protocol for electroporation of grown t/pos bacteria from Bio-Rad’s website prof. T will print and I’ll place here.
-The mycobacterium will be done by electroporation protocol on pg 15.
+Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
-) First I placed a Cuvette into Ice bath and thawed electro comp cells
+==9/24/10 ==
-) I set up 5 1.5 mL Sterile Centrifuge tubes  with 900 mL SOB media
-) we will electroporate Irene’s electrocomp cells w/PG10 and mine will contain the parts after electroporation.
-Code
+Today I’m testing absorbance of our trials @ 395nm
-AT – A.tumefacions SL – S.lactis BM – B.magetanium MP – Mycobacteria
-BT – B.thurigensis
-split cells using 40 mL Hy part my cells then electroporate
+== 9/28/10 ==
-I electroporated all except mycobacteria now letting cells sit for at least
+Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
-Just added 1 mL to electroporated Cultures they had sat for 20 min before adding.
-- Now I will electroporate mycobacteria using E – Coli protocol from pg 15 using 40 mL cells 4 mL pg10 Electroporation hit twice Read out 1.8 kv @ 5.2 ms then 1.79 kv @ 5.1 ms. then placed into 900 mL SOB media then incubate @ 30 degrees C for 3 hrs.
-Results
+== 9/30/10==
-From this we found that agrobacteria will be the best host for our beast/IGem
+LacZ without GFP also with RBS and terminator
-we will do further testing towards our ultimate goal.
-== 9/21/10 ==
+==10/1/10==
+electrophoresis
-	Today we will electroporate  Agrobacterium with part T9002 using protocol from pg  22 – 23
+==10/5/10==
-) I will put frozen electro comp cells from -80 degrees C freezer
+pTet GFP
-) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL
-) Icing Cuvette (.1) and now prepared to electroporate
-) Reading for 20 mL was 2.2 kv @ 0.70 ms
-) then removed with 1 mL SOB media
-) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs prof T. will streak.
+==10/7/10==
-== 9/22/10 ==
+Electroporating 2 parts with E-coli bacteria
+==10/8/10==
-	Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
-== 9/22/10 ==
+LB-Lennox Broth & Electroporation
+==10/12/10==
+hold until Friday
-	The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
+==10/13/10==
+Discoveries!
-== 9/23/10 ==
+==10/15/10==
+Using openwetware.org protocol
-	 Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
+B-galactosidase Assay
-==9/24/10 ==
+BBL Trypticase soy Broth and Agar
+DPBS
-	Today I’m testing absorbance of our trials @ 395nm
+== 10/19/10==
+Protocol-- Electroporation man for E-Coli
+== 10/21/10 ==
+High Efficiency Electrotransformation of E.coli
-== 9/28/10 ==
+== 10/25/10 ==
-	Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
+DNA from BlueGuy
+== 10/26/10 ==
+Final steps pack and ship
@@ Line 221: / Line 252: @@
 |-
-|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
+|align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
 |-
 |
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 |
-|align="center"|[[Team:IvyTech-South_Bend | Team Example]]
+|
 |}

Team:IvyTech-South Bend/Notebook

From 2010.igem.org

Latest revision as of 00:44, 28 October 2010

Contents

Notebook

IGEM Part #s and Uses

6/28/10

6/30/10

7/28/10

8/24/10

8/27/10

8/27/10

8/31/10

8/31/10

9/1/10

9/2/10

9/2/10

9/3/10

9/9/10

9/10/10

9/14/10

9/15/10

9/16/10

9/17/10

10/18/10

9/21/10

9/22/10

9/22/10

9/23/10

9/24/10

9/28/10

9/30/10

10/1/10

10/5/10

10/7/10

10/8/10

10/12/10

10/13/10

10/15/10

10/19/10

10/21/10

10/25/10

10/26/10