Team:MIT uvexp

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  <li><a href="https://2010.igem.org/Team:MIT_bact">Bacterial Protocol</a><br></li>
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    <ul><li><a href="https://2010.igem.org/Team:MIT_bconst">Biobrick Construction</a><br></li>
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        <li><a href="https://2010.igem.org/Team:MIT_bexp">Bacterial Experiments</a></li></ul>
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  <li><a href="https://2010.igem.org/Team:MIT_mmethods">Mammalian Protocol</a></li>
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    <ul><li><a href="https://2010.igem.org/Team:MIT_mmethods">Microfluidics</a></li>
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        <li><a href="https://2010.igem.org/Team:MIT_mge">Genetic Engineering</a></li>
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        <li><a href="https://2010.igem.org/Team:MIT_gateway">Gateway Cloning</a></li>
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<table width=70%><tr><td><div id="bodybaby">uv exposure setup</div></td></table>
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<table width=70%><tr><td><div class="bodybaby">bacterial experimental setup</div></td>
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Grow cell lawn in M9 minimal top agar. Keep out of the light. Remove top of petri dish, and cover cells with mask that exposes parts of the agar.<br><a href="https://static.igem.org/mediawiki/2009/2/2e/Mask.jpg" class="thickbox" title="Cover cell lawn with mask."><img width=200px src="https://static.igem.org/mediawiki/2009/2/2e/Mask.jpg"></a><br>
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Put masked cells into apparatus that ensures there will be no UV exposure to the sides of the plate, and minimizes reflection of the UV from the bottom of the plate.<br><a href="https://static.igem.org/mediawiki/2009/c/c8/Apparatus.jpg" class="thickbox" title="Minimize artifacts by blocking sideways or reflected UV exposure to the agar."><img width=200px src="https://static.igem.org/mediawiki/2009/c/c8/Apparatus.jpg"></a><br>
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Expose cells to predetermined power of UV exposure, remove petri dish, recover, and reincubate.
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Latest revision as of 01:58, 19 October 2010



bacterial experimental setup

Grow cell lawn in M9 minimal top agar. Keep out of the light. Remove top of petri dish, and cover cells with mask that exposes parts of the agar.

Put masked cells into apparatus that ensures there will be no UV exposure to the sides of the plate, and minimizes reflection of the UV from the bottom of the plate.

Expose cells to predetermined power of UV exposure, remove petri dish, recover, and reincubate.

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