Latest revision as of 19:32, 10 December 2010

Home	Team	Project	Protocols	Notebook	Judging Criteria	Attributions	Safety

Welcome!

Welcome to the Team Minnesota Wiki for iGEM 2010!

We are a team of undergraduate students along with many faculty and post-doc advisors. This is the third year that Minnesota has sent a team to iGEM.

Team Minnesota take won a gold medal and the Best Natural Part Special Prize at iGEM 2010!!

Metabolic Engineering: In vivo Nanobioreactors

Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our iGEM project, we have cloned (and expressed in E. coli) Salmonella LT2 genes responsible for the production and assembly of ethanolamine utilization microcompartments. Additionally, we have determined a signal sequence that targets an ethanolamine utilization enzyme to the microcompartment and verified this by fusing the sequence to GFP and observing that this causes the GFP reporter to localize to the compartment. We conclude that recombinant microcompartments housing targeted enzymes can function as in vivo bioreactors with high reaction efficiencies.

Highlights for the Judges

Judges! Please navigate to our Judging Criteria page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.

Sponsers

@@ Line 1: / Line 1: @@
-{|align="center" cellpadding="25"
+[[Image:mnlogo.jpg|300px|center|frame]]
-|[[Image:mnlogo.jpg|300px|center|frame]]
-|}
 {| style="color:gold;background-color:#800000;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="90%" align="center"
@@ Line 7: / Line 5: @@
 !align="center"|[[Team:Minnesota/Team|<font color="gold">Team</font>]]
 !align="center"|[[Team:Minnesota/Project|<font color="gold">Project</font>]]
-!align="center"|[[Team:Minnesota/Designer|<font color="gold">Protocols</font>]]
+!align="center"|[[Team:Minnesota/Protocols|<font color="gold">Protocols</font>]]
-!align="center"|[[Team:Minnesota/Modeling|<font color="gold">#</font>]]
+!align="center"|[[Team:Minnesota/Notebook|<font color="gold">Notebook</font>]]
-!align="center"|[[Team:Minnesota/Notebook|<font color="gold">Judging Criteria</font>]]
+!align="center"|[[Team:Minnesota/Judging|<font color="gold">Judging Criteria</font>]]
-!align="center"|[[Team:Minnesota/Parts Characterization|<font color="gold">Competition Requirements</font>]]
+!align="center"|[[Team:Minnesota/Attributions|<font color="gold">Attributions</font>]]
+!align="center"|[[Team:Minnesota/Safety|<font color="gold">Safety</font>]]
 |}
 {|align="justify"
@@ Line 16: / Line 15: @@
 Welcome to the Team Minnesota Wiki for iGEM 2010!
-We are a team of undergraduate and graduate students along with many advisors.  This is the third year that Minnesota has sent a team to iGEM. Check back later for more updates!
+We are a team of undergraduate students along with many faculty and post-doc advisors.  This is the third year that Minnesota has sent a team to iGEM.
+[[Image:MN Igem10 team.jpg|center]]
+Team Minnesota take won a gold medal and the Best Natural Part Special Prize at iGEM 2010!!
-<h2>The Mission</h2>
-This year our team is pursuing two projects, one experimental and one computational. We are very excited about the potential of each of these projects. Check out our notebook and other pages for more details!
 <b>Metabolic Engineering: In vivo Nanobioreactors</b>
-Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells.  Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates.  For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment.  Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes.  To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli.
+Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our iGEM project, we have cloned (and expressed in E. coli) Salmonella LT2 genes responsible for the production and assembly of ethanolamine utilization microcompartments. Additionally, we have determined a signal sequence that targets an ethanolamine utilization enzyme to the microcompartment and verified this by fusing the sequence to GFP and observing that this causes the GFP reporter to localize to the compartment. We conclude that recombinant microcompartments housing targeted enzymes can function as in vivo bioreactors with high reaction efficiencies.
-<b>Athina Software Package</b>
-<b>A</b>utomated <b>Th</b>eoretical <b>I</b>nteraction <b>N</b>etwork <b>A</b>ssembly is a web service to transform a sequence of BioBricks, or any other set of biomolecular components, to a set of reactions that can be simulated dynamically. The user simply inputs the Biobricks and their relationships and Designer builds a reaction network using biological interactions rules. This software package also includes a wiki. This is a web service to collect the kinetic parameters necessary to create a model that can be simulated.
 <h2>Highlights for the Judges</h2>
-Judges! Please navigate to our [https://2010.igem.org/Team:Minnesota/Criteria Judging Criteria] page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.
+Judges! Please navigate to our [[Team:Minnesota/Judging|Judging Criteria]] page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.
+{|align="center" cellpadding="25"
-[[Image:teamphoto2.jpeg|400px|right]]
+|[[Image:UMNSponsors.png|300px|center|frame|Sponsers]]
-<!--- The Mission, Experiments --->