Team:Edinburgh/Notebook/Blue light producer

From 2010.igem.org

(Difference between revisions)

Latest revision as of 02:12, 28 October 2010

These notes correspond to the part of the project detailed here.

Blue Light Producer

5/7/2010

LuxAB & LuxCDE operon agarose gel
Lane1: About2.1.kbp
Lane 2: The highest band- very faint- about 3.5kbp

13/7/2010

Empty edinbrick vector for control.

LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
Still have some digested LuxAB-ediI so re-did ligation and transformation.

Lane 1&15	Lane 2	Others
Ladder	EdiI	LuxAB

Lane 1	Lane 2 +3	Lanes 3-6
Ladder	EdiI	LuxAB

29/7/2010

The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

4/8/2010

made minipreps of blue (negative colonies)- double digest and ran in comparison with pure edinbrickI
LacZ band dissapears but colonies still grow blue (WTF)
Has bands equaling to luxAB and EdiI but there was no glowing

12/8/2010 update on LumP:

still getting pink/red colonies from transformants
single digests give a band of about 3kb
double digests give a band of about 2.5 kb -highly confusing
and also, no sign on RFP despite red/pink growth.
Vector wa ssequenced and LumP is definately there.

Lanes 2-5: single digests of lumP Lane 6: double digest of lumP

26/8/2010

Marker (Lane 1), minipreps 6-10 luxABlumP (lanes 2-6)

single digests of LuxABlumP

7/9/2010

minipreps of luxCDE: samples 1&4 usable

Lane 1	Lane 2-6	Lanes 7
Ladder	luxCDE00:1-5 minipreps	Ladder

@@ Line 22: / Line 22: @@
 <div id="nav">
-<ul class="dropdown dropdown-horizontal">
+<ul class="dropdown dropdown-horizontal" style="background: none;">
   <li><a href="https://2010.igem.org/Team:Edinburgh" class="dir">home</a>
@@ Line 43: / Line 43: @@
     <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">future work</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">the future</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li>
    </ul>
@@ Line 50: / Line 50: @@
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
    <ul>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the repressilator</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the project</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li>
@@ Line 59: / Line 59: @@
     <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">future work</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">the future</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li>
    </ul>
@@ Line 70: / Line 70: @@
     <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Tools">tools</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">future work</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">the future</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li>
    </ul>
@@ Line 78: / Line 79: @@
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
    <ul>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Aspects">human aspects</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Branding">iGEM survey</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li>
-    <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Future">future work</a></li>
+    <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">further thoughts</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li>
    </ul>
@@ Line 89: / Line 92: @@
    <ul>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Collaboration">collaboration</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Attribution">attribution</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/BRIDGE">BRIDGE</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_producer">red light</a></li>
@@ Line 94: / Line 98: @@
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_producer">blue light</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_sensor">blue sensor</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Green_light_producer">green light</a></li>
+   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Green_light_sensor">green sensor</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Modelling">modelling</a></li>
     <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Safety">safety</a></li>
@@ Line 109: / Line 115: @@
 <br>
 <br>
+<div id="windowbox" style="border: .2em solid #660000; padding: 5px; position:fixed; top:50%; right:30px; width:8%;">
+<span style="color:ivory;">These <b>notes</b> correspond to the part of the <b>project</b> detailed <a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Blue_light_producer">here</a>.</span>
+</div>
 </html>
@@ Line 114: / Line 125: @@
 == Blue Light Producer ==
 <br>
+'''5/7/2010'''
+*LuxAB & LuxCDE operon agarose gel
+* Lane1: About2.1.kbp
+* Lane 2: The highest band- very faint- about 3.5kbp
+[[Image:150.JPG]]
+'''13/7/2010'''
+Empty edinbrick vector for control.
+* LuxAB + edinbrick ligation.
+'''28/7/2010'''
+an update on LuxAB:
+*The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
+*Still have some digested LuxAB-ediI so re-did ligation and transformation.
+[[Image:154.JPG]]
+{| border="1"
+| Lane 1&15
+| Lane 2
+| Others
+|-
+| Ladder
+| EdiI
+| LuxAB
+|}
+[[Image:155.JPG]]
+{| border="1"
+| Lane 1
+| Lane 2 +3
+| Lanes 3-6
+|-
+| Ladder
+| EdiI
+| LuxAB
+|}
+'''29/7/2010'''
+[[Image:156.jpg]]
+* The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working
+'''4/8/2010'''
+* made minipreps of blue (negative colonies)- double digest and ran in comparison with pure edinbrickI
+* LacZ band dissapears but colonies still grow blue (WTF)
+* Has bands equaling to luxAB and EdiI but there was no glowing
+[[Image:162.jpg]]
+'''12/8/2010'''
+update on LumP:
+* still getting pink/red colonies from transformants
+* single digests give a band of about 3kb
+* double digests give a band of about 2.5 kb -highly confusing
+* and also, no sign on RFP despite red/pink growth.
+* Vector wa ssequenced and LumP is definately there.
+[[Image:160.jpg]]
+Lanes 2-5: single digests of lumP
+Lane 6: double digest of lumP
+'''26/8/2010'''
+* Marker (Lane 1), minipreps 6-10 luxABlumP (lanes 2-6)
+[[Image:129.JPG]]
+* single digests of LuxABlumP
+[[Image:131.JPG]]
+'''7/9/2010'''
+*minipreps of luxCDE: samples 1&4 usable
+{| border="1"
+| Lane 1
+| Lane 2-6
+| Lanes 7
+|-
+| Ladder
+| luxCDE00:1-5 minipreps
+| Ladder
+|}
+[[Image:140.JPG]]