Team:Washington

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<!---------------------------------------PAGE CONTENT GOES BELOW THIS---------------------------------------->
 
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=== Antibiotics for the 21st Century ===
 
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While vital to our quality of life, traditional antibiotics face the serious
While vital to our quality of life, traditional antibiotics face the serious
problems of widespread bacterial resistance and destruction of natural gut
problems of widespread bacterial resistance and destruction of natural gut
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the immune system.  In our second project, we re-engineered and transplanted
the immune system.  In our second project, we re-engineered and transplanted
a protein secretion system capable of combating Gram-negative bacteria into
a protein secretion system capable of combating Gram-negative bacteria into
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E. coli.  This system was designed to target Gram-negative pathogens in a
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''E. coli''.  This system was designed to target Gram-negative pathogens in a
modular and controllable fashion.  These two systems are the vanguard of a
modular and controllable fashion.  These two systems are the vanguard of a
new era of antibiotics using the power of nature harnessed with the tools of
new era of antibiotics using the power of nature harnessed with the tools of
synthetic biology.
synthetic biology.
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'''will include intro image, idea is currently being fleshed out from sketch into image'''
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'''[[Team:Washington/Gram_Negative|Gram(-) Therapeutic]]'''
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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'''[[Team:Washington/Gram_Positive|Gram(+) Therapeutic]]'''
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=21st century antibiotics GENERAL STORYLINE FLOW=
 
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'''The bullet points are the key images that need to be generated.  They should be very general and easy to understand!  The text will basically be explaning the pictures and telling the story.... think of this as a comic book!
 
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==gram(+)==
 
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===summary===
 
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*why anthrax is bad
 
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*proteins fighting anthrax
 
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===design===
 
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*circular permutation
 
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*computational design
 
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===build===
 
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*How we made mutants
 
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*How we made protiein
 
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===Test===
 
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*gel/mass spec cp vs D
 
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*activity cp vs D
 
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*catalytic KO of CP vs CP
 
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*CP mutations
 
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*Specificity switch
 
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===Future Directions===
 
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*Just some bullet points, no figures needed
 
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==gram(-)==
 
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puzzle bobble with evil bacteria as bubbles?
 
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===summary (bugs vs bugs)===
 
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*type 6 secretion (spear) done-Matthew Harger
 
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*Toxin/Antitoxin produced in prescence of XXX (poison) done
 
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===design===
 
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*operon in Pseudomonas —> operon in e coli ( fosmid image)
 
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**what parts of this operon are needed? justin has this
 
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*sensor —produces—> toxin/antitoxin (classic tinkercell picture) done
 
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===build===
 
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*exporter —> recombineering to promoter  image already made
 
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*sensor/toxin: —> PCR —> HSL biobrick vector made by Matthew Harger
 
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===Test===
 
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*Exporter:  Still under construction
 
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*Sensor/Toxin
 
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**Toxin is toxic in ecoli: (Rachael hood,2010) paper;  Sensor works and produces tse2 as expected ( include image of western blot with annotations)
 
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Antidote still works in e.coli indirect evidence, state lack of mutations, may not need image.;
 
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===Future Directions===
 
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*Just some quick Bullet Points
 
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<!---------------------------------------PAGE CONTENT GOES ABOVE THIS---------------------------------------->
 
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<div style="text-align:left">
 
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'''[[Team:Washington/Project/Baker|Gram(+) Therapeutic]]'''
 
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<div style="text-align:right">
 
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'''[[Team:Washington/Project/Mougous|Gram(-) Therapeutic]]'''
 
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Latest revision as of 01:14, 28 October 2010

21stCenturyAntibioticsBanner START button Player 1 Gram(-) Player 2 Gram(+) Gram(+) Side Gram(-) Side


While vital to our quality of life, traditional antibiotics face the serious problems of widespread bacterial resistance and destruction of natural gut flora - problems which call for improved twenty-first century antibiotics. Using synthetic biology tools, we designed, built, and tested two new systems to fight infections by both broad types of bacteria - Gram-positive and Gram-negative. Our first project targets Bacillus anthracis, the Gram-positive pathogen that causes anthrax. We re-engineered an enzyme to remove the pathogen's protective coating, rendering it defenseless against the immune system. In our second project, we re-engineered and transplanted a protein secretion system capable of combating Gram-negative bacteria into E. coli. This system was designed to target Gram-negative pathogens in a modular and controllable fashion. These two systems are the vanguard of a new era of antibiotics using the power of nature harnessed with the tools of synthetic biology.


Gram(-) Therapeutic                  Gram(+) Therapeutic


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