Team:Washington

From 2010.igem.org

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<img src="https://static.igem.org/mediawiki/2010/b/be/Uwb.gif" alt="21stCenturyAntibioticsBanner" usemap="#bannermap" />
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<area shape="rect" alt="START button" title="Explore the Wiki" coords="384,258,447,279" href="https://2010.igem.org/Team:Washington/Gram_Negative"/>
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<area shape="rect" alt="Player 1 Gram(-)" title="Gram(-) Therapeutic" coords="343,281,493,300" href="https://2010.igem.org/Team:Washington/Gram_Negative"/>
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<area shape="rect" alt="Player 2 Gram(+)" title="Gram(+) Therapeutic" coords="343,302,492,320" href="https://2010.igem.org/Team:Washington/Gram_Positive"/>
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<area shape="poly" alt="Gram(+) Side" title="Gram(+) Therapeutic" coords="329,269,326,319,279,350,5,349,5,6,165,6,146,88,165,99,170,131,139,164,140,197,174,211" href="https://2010.igem.org/Team:Washington/Gram_Positive"/>
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<area shape="poly" alt="Gram(-) Side" title="Gram(-) Therapeutic" coords="507,269,509,322,546,350,793,350,793,3,689,4,673,23,681,76,660,116,648,168,655,197,623,248" href="https://2010.igem.org/Team:Washington/Gram_Negative"/>
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<!---------------------------------------PAGE CONTENT GOES BELOW THIS---------------------------------------->
 
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'''Note: this is a very crude sketch that will be (likely) turned into a final banner'''
 
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[[Image:Washington_main_Image_test.png]]
 
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=== Antibiotics for the 21st Century ===
 
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While vital to our quality of life, traditional antibiotics face the serious
While vital to our quality of life, traditional antibiotics face the serious
problems of widespread bacterial resistance and destruction of natural gut
problems of widespread bacterial resistance and destruction of natural gut
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the immune system.  In our second project, we re-engineered and transplanted
the immune system.  In our second project, we re-engineered and transplanted
a protein secretion system capable of combating Gram-negative bacteria into
a protein secretion system capable of combating Gram-negative bacteria into
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E. coli.  This system was designed to target Gram-negative pathogens in a
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''E. coli''.  This system was designed to target Gram-negative pathogens in a
modular and controllable fashion.  These two systems are the vanguard of a
modular and controllable fashion.  These two systems are the vanguard of a
new era of antibiotics using the power of nature harnessed with the tools of
new era of antibiotics using the power of nature harnessed with the tools of
synthetic biology.
synthetic biology.
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'''will include intro image, idea is currently being fleshed out from sketch into image'''
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story: (img for each point)
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*21st century antibiotics, needs a banner to show what it means? 12th century battle scene with bacteria?
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*postive and negative
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'''[[Team:Washington/Gram_Negative|Gram(-) Therapeutic]]'''
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*summary(pacman graphic), why anthrax bad? proteins fighting anthrax.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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*Design
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'''[[Team:Washington/Gram_Positive|Gram(+) Therapeutic]]'''
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*(CP graphic)
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</center>
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*computational redesign
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*build
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*mutants
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*expression and purification
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*test result
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*gel/mass spec cp vs D
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*activity cp vs D
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*catalytic KO of CP vs CP
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*CP mutations
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*Specificity switch
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banner
 
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gram(+)
 
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*summary
 
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**why anthrax is bad
 
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**proteins fighting anthrax
 
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*design
 
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**circular permutation
 
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**computational design
 
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*build
 
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**mutants
 
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*test
 
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**lots here
 
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gram(-)
 
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*summary (bugs vs bugs)
 
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**type 6 secretion (poison spear)
 
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**target (make system that goes after Z)
 
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*design
 
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**operon in Pseudomonas —> operon in e coli
 
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**what we need
 
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**sensor —> toxin/antitoxin
 
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*build
 
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**exporter —> recombineering to promoter
 
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**sensor/toxin —> PCR —> HSL biobrick vector
 
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<!---------------------------------------PAGE CONTENT GOES ABOVE THIS---------------------------------------->
 
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<div style="text-align:left">
 
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'''[[Team:Washington/Project/Baker|Gram(+) Therapeutic]] &rarr;'''
 
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<div style="text-align:right">
 
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'''[[Team:Washington/Project/Mougous|Gram(-) Therapeutic]] &rarr;'''
 
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{{Template:Team:Washington/Templates/Footer}}
{{Template:Team:Washington/Templates/Footer}}

Latest revision as of 01:14, 28 October 2010

21stCenturyAntibioticsBanner START button Player 1 Gram(-) Player 2 Gram(+) Gram(+) Side Gram(-) Side


While vital to our quality of life, traditional antibiotics face the serious problems of widespread bacterial resistance and destruction of natural gut flora - problems which call for improved twenty-first century antibiotics. Using synthetic biology tools, we designed, built, and tested two new systems to fight infections by both broad types of bacteria - Gram-positive and Gram-negative. Our first project targets Bacillus anthracis, the Gram-positive pathogen that causes anthrax. We re-engineered an enzyme to remove the pathogen's protective coating, rendering it defenseless against the immune system. In our second project, we re-engineered and transplanted a protein secretion system capable of combating Gram-negative bacteria into E. coli. This system was designed to target Gram-negative pathogens in a modular and controllable fashion. These two systems are the vanguard of a new era of antibiotics using the power of nature harnessed with the tools of synthetic biology.


Gram(-) Therapeutic                  Gram(+) Therapeutic


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