Latest revision as of 07:54, 27 October 2010

Electrophoresis of pSB1C3 concentrated to 50 ug/uL

Electrophoresis of concentrated pSB1C3

Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed

Lane	DNA
１	Added too much of marker, mistake
２	λ/Hind III & EcoR I
３	pSB1C3 solution
４	pSB1C3 solution

IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
Only band visible was monomer（about 2000 bp）

Remaining amount from check via electrophoresis，namely 49 uL was filtrated with Microcon YM-10

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-=[[アガロースゲル電気泳動|Electrophoresis]] of pSB1C3 concentrated to 50 ug/uL=
+=Electrophoresis of pSB1C3 concentrated to 50 ug/uL=
 [[Image:HokkaidoU Japan 20100826a.jpg‎|200px|right|thumb|Electrophoresis of concentrated pSB1C3]]
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 |-
 |２
-|[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png Lambda/''Hin''d III & EcoR I]
+|[https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png λ/''Hin''d III & EcoR I]
 |-
 |３
@@ Line 29: / Line 29: @@
 * Only band visible was monomer（about 2000 bp）
-=pSB1A3, pSB1C3, pSB1K3のPCR solutionろ過=
+=Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions=
-電気泳動で確かめた残り，49 uLをMicrocon YM-10でろ過した．
+Remaining amount from check via electrophoresis，namely 49 uL was filtrated with  Microcon YM-10